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On-chip determination of C-reactive protein using magnetic particles in continuous flow

Phurimsak, Chayakom; Tarn, Mark D.; Peyman, Sally A.; Greenman, John; Pamme, Nicole

Authors

Chayakom Phurimsak

Mark D. Tarn

Sally A. Peyman

Abstract

We demonstrate the application of a multilaminar flow platform, in which functionalized magnetic particles are deflected through alternating laminar flow streams of reagents and washing solutions via an external magnet, for the rapid detection of the inflammatory biomarker, C-reactive protein (CRP). The two-step sandwich immunoassay was accomplished in less than 60 s, a vast improvement on the 80−300 min time frame required for enzyme-linked immunosorbent assays (ELISA) and the 50 min necessary for off-chip magnetic particle-based assays. The combination of continuous flow and a stationary magnet enables a degree of autonomy in the system, while a detection limit of 0.87 μg mL−1 makes it suitable for the determination of CRP concentrations in clinical diagnostics. Its applicability was further proven by assaying real human serum samples and comparing those results to values obtained using standard ELISA tests.

Publication Date Nov 4, 2014
Journal Analytical chemistry
Print ISSN 0003-2700
Electronic ISSN 1520-6882
Publisher American Chemical Society
Peer Reviewed Peer Reviewed
Volume 86
Issue 21
Pages 10552-10559
Institution Citation Phurimsak, C., Tarn, M. D., Peyman, S. A., Greenman, J., & Pamme, N. (2014). On-chip determination of C-reactive protein using magnetic particles in continuous flow. Analytical chemistry, 86(21), 10552-10559. https://doi.org/10.1021/ac5023265
DOI https://doi.org/10.1021/ac5023265
Keywords C-reactive protein
Publisher URL http://pubs.acs.org/doi/full/10.1021/ac5023265
Additional Information This document is the Accepted Manuscript version of a Published Work that appeared in final form in Analytical chemistry, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see [insert ACS Articles on Request author-directed link to Published Work, see http://pubs.acs.org/pag...sonrequest/index.html].

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