On-chip determination of C-reactive protein using magnetic particles in continuous flow

Phurimsak, Chayakom; Tarn, Mark D.; Peyman, Sally A.; Greenman, John; Pamme, Nicole

Abstract

We demonstrate the application of a multilaminar flow platform, in which functionalized magnetic particles are deflected through alternating laminar flow streams of reagents and washing solutions via an external magnet, for the rapid detection of the inflammatory biomarker, C-reactive protein (CRP). The two-step sandwich immunoassay was accomplished in less than 60 s, a vast improvement on the 80−300 min time frame required for enzyme-linked immunosorbent assays (ELISA) and the 50 min necessary for off-chip magnetic particle-based assays. The combination of continuous flow and a stationary magnet enables a degree of autonomy in the system, while a detection limit of 0.87 μg mL−1 makes it suitable for the determination of CRP concentrations in clinical diagnostics. Its applicability was further proven by assaying real human serum samples and comparing those results to values obtained using standard ELISA tests.

Citation

Phurimsak, C., Tarn, M. D., Peyman, S. A., Greenman, J., & Pamme, N. (2014). On-chip determination of C-reactive protein using magnetic particles in continuous flow. Analytical chemistry, 86(21), 10552-10559. https://doi.org/10.1021/ac5023265

• Acceptance Date: Oct 2, 2014
• Online Publication Date: Oct 2, 2014
• Publication Date: Nov 4, 2014
• Deposit Date: Jan 6, 2015
• Publicly Available Date: Jan 6, 2015
• Journal: Analytical chemistry
• Print ISSN: 0003-2700
• Publisher: American Chemical Society
• Peer Reviewed: Peer Reviewed
• Volume: 86
• Issue: 21
• Pages: 10552-10559
• DOI: https://doi.org/10.1021/ac5023265
• Keywords: C-reactive protein
• Public URL: https://hull-repository.worktribe.com/output/371583
• Publisher URL: http://pubs.acs.org/doi/full/10.1021/ac5023265
• Additional Information: This document is the Accepted Manuscript version of a Published Work that appeared in final form in Analytical chemistry, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see [insert ACS Articles on Request author-directed link to Published Work, see http://pubs.acs.org/page/policy/articlesonrequest/index.html].
• Contract Date: Jan 6, 2015