Skip to main content

Research Repository

Advanced Search

Multiplex sorting of foodborne pathogens by on-chip free-flow magnetophoresis

Ngamsom, Bongkot; Esfahani, Mohammad M.N.; Phurimsak, Chayakom; Lopez-Martinez, Maria J.; Raymond, Jean-Claude; Broyer, Patrick; Patel, Pradip; Pamme, Nicole

Authors

Bongkot Ngamsom

Mohammad M.N. Esfahani

Chayakom Phurimsak

Maria J. Lopez-Martinez

Jean-Claude Raymond

Patrick Broyer

Pradip Patel

Nicole Pamme



Abstract

This study reports multiplex sorting of Salmonella typhimurium and Escherichia coli 0157, from broth cultures and from pathogen-spiked skinned chicken breast enrichment broths by employing microfluidic free-flow magnetophoresis. Magnetic beads of different sizes and magnetite content, namely Dynabeads anti-salmonella and Hyglos-Streptavidin beads together with the corresponding pathogen-specific biotinylated recombinant phages, were utilised as affinity solid phases for the capture and concentration of viable S. typhimurium and E. coli 0157. Following optimisation, the protocol was used to demonstrate continuous magnetophoretic sorting of the two pathogen-bound magnetic bead populations from mixed cultures and from pathogen-spiked chicken pre-enrichment broths under the influence of a Halbach magnet array. For example, in the la tter case, a pure population of S. typhimurium-bound Dynabeads (72% recovery) was sorted from a 100 μL mixture containing E. coli 0157-bound Hyglos beads (67% recovery) within 1.2 min in the presence of 0.1% Tween 20. This proof-of-principle study demonstrates how more than one pathogen type can be simultaneously isolated/enriched from a single food pre-enrichment broth (e.g. Universal food enrichment broth).

Citation

Ngamsom, B., Esfahani, M. M., Phurimsak, C., Lopez-Martinez, M. J., Raymond, J., Broyer, P., …Pamme, N. (2016). Multiplex sorting of foodborne pathogens by on-chip free-flow magnetophoresis. Analytica Chimica Acta, 918, 69-76. https://doi.org/10.1016/j.aca.2016.03.014

Journal Article Type Article
Acceptance Date Mar 7, 2016
Online Publication Date Mar 18, 2016
Publication Date Apr 28, 2016
Deposit Date Mar 22, 2016
Publicly Available Date Mar 22, 2016
Journal Analytica chimica acta
Print ISSN 0003-2670
Electronic ISSN 1873-4324
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 918
Pages 69-76
DOI https://doi.org/10.1016/j.aca.2016.03.014
Keywords Multiplex sorting, Salmonella & E.coli 0157, Food enrichment broth, Free-flow magnetophoresis, Affinity magnetic separation
Public URL https://hull-repository.worktribe.com/output/434001
Publisher URL http://www.sciencedirect.com/science/article/pii/S0003267016303312
Additional Information This article is maintained by: Elsevier; Article Title: Multiplex sorting of foodborne pathogens by on-chip free-flow magnetophoresis; Journal Title: Analytica Chimica Acta; CrossRef DOI link to publisher maintained version: http://dx.doi.org/10.1016/j.aca.2016.03.014; Content Type: article; Copyright: Copyright © 2016 Elsevier B.V. All rights reserved.

Files





You might also like



Downloadable Citations