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Exposure to low pH induces molecular level changes in the marine worm, Platynereis dumerilii

Hardege, Jorg; Wäge, Janine; Lerebours, Adelaide; Rotchell, Jeanette; Hardege, Jörg D.; Rotchell, Jeanette M.

Authors

Janine Wäge

Adelaide Lerebours

Abstract

© 2015 Elsevier Inc. Fossil fuel emissions and changes in net land use lead to an increase in atmospheric CO 2 concentration and a subsequent decrease of ocean pH. Noticeable effects on organisms' calcification rate, shell structure and energy metabolism have been reported in the literature. To date, little is known about the molecular mechanisms altered under low pH exposure, especially in non-calcifying organisms. We used a suppression subtractive hybridisation (SSH) approach to characterise differentially expressed genes isolated from Platynereis dumerilii, a non-calcifying marine polychaeta species, kept at normal and low pH conditions. Several gene sequences have been identified as differentially regulated. These are involved in processes previously considered as indicators of environment change, such as energy metabolism (NADH dehydrogenase, 2-oxoglutarate dehydrogenase, cytochrome c oxidase and ATP synthase subunit F), while others are involved in cytoskeleton function (paramyosin and calponin) and immune defence (fucolectin-1 and paneth cell-specific alpha-defensin) processes. This is the first study of differential gene expression in a non-calcifying, marine polychaete exposed to low pH seawater conditions and suggests that mechanisms of impact may include additional pathways not previously identified as impacted by low pH in other species.

Publication Date Feb 1, 2016
Journal Ecotoxicology and environmental safety
Print ISSN 0147-6513
Electronic ISSN 1090-2414
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 124
Issue February
Pages 105-110
Institution Citation Wäge, J., Lerebours, A., Hardege, J. D., & Rotchell, J. M. (2016). Exposure to low pH induces molecular level changes in the marine worm, Platynereis dumerilii. Ecotoxicology and environmental safety, 124(February), 105-110. https://doi.org/10.1016/j.ecoenv.2015.10.008
DOI https://doi.org/10.1016/j.ecoenv.2015.10.008
Keywords pH; Acidification; Polychaete; Gene expression
Publisher URL http://www.sciencedirect.com/science/article/pii/S014765131530124X
Copyright Statement © 2016, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/
Additional Information Authors' accepted manuscript of article which has been published in: Ecotoxicology and environmental safety, 2016, v.124.

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Copyright Statement
© 2016, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/




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