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Effects of low seawater pH on the marine polychaete Platynereis dumerilii

Hardege, Joerg D.; Waege, Janine; Wäge, Janine; Hardege, Jörg D.; Larsson, Tomas A.; Simakov, Oleg; Chapman, Emma C.; Arendt, Detlev; Rotchell, Jeanette M.

Authors

Joerg D. Hardege

Janine Waege

Janine Wäge

Tomas A. Larsson

Oleg Simakov

Emma C. Chapman

Detlev Arendt



Abstract

An important priority for any organism is to maintain internal cellular homeostasis including acidbase balance. Yet, the molecular level impacts of changing environmental conditions, such as low pH, remain uncharacterised. Herein, we isolate partial Na+/H+ exchangers (NHE), carbonic anhydrase (CA), and calmodulin (CaM) genes from a polychaete, Platynereis dumerilii and investigate their relative expression in acidified seawater conditions. mRNA expression of NHE was significantly down-regulated after 1 h and up-regulated after 7 days under low pH treatment (pH 7.8), indicating changes in acid-base transport. Furthermore, the localisation of NHE expression was also altered. A trend of down regulation in CA after 1 h was also observed, suggesting a shift in the CO2 and HCO3- balance. No change in CaM expression was detected after 7 days exposure to acidified seawater. This study provides insight into the molecular level changes taking place following exposure to acidified seawater in a non-calcifying, ubiquitous, organism.

Citation

Wäge, J., Hardege, J. D., Larsson, T. A., Simakov, O., Chapman, E. C., Arendt, D., & Rotchell, J. M. (2015). Effects of low seawater pH on the marine polychaete Platynereis dumerilii. Marine pollution bulletin, 95(1), 166-172. https://doi.org/10.1016/j.marpolbul.2015.04.027

Journal Article Type Article
Acceptance Date Apr 11, 2015
Online Publication Date Apr 22, 2015
Publication Date Jun 15, 2015
Deposit Date Apr 14, 2015
Publicly Available Date Apr 22, 2015
Journal Marine pollution bulletin
Print ISSN 0025-326X
Electronic ISSN 1879-3363
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 95
Issue 1
Pages 166-172
DOI https://doi.org/10.1016/j.marpolbul.2015.04.027
Keywords Acid-base regulation, Low pH, Gene expression, Polychaeta, Platynereis dumerilii
Public URL https://hull-repository.worktribe.com/output/372383
Publisher URL http://www.sciencedirect.com/science/article/pii/S0025326X15002295
Additional Information This article is maintained by: Elsevier; Article Title: Effects of low seawater pH on the marine polychaete Platynereis dumerilii; Journal Title: Marine Pollution Bulletin; CrossRef DOI link to publisher maintained version: http://dx.doi.org/10.1016/j.marpolbul.2015.04.027; Content Type: article; Copyright: Copyright © 2015 Elsevier Ltd. All rights reserved.

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Publisher Licence URL
http://creativecommons.org/licenses/by-nc-nd/4.0/

Copyright Statement
© 2016, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/



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