Effects of low seawater pH on the marine polychaete Platynereis dumerilii

Wäge, Janine; Hardege, Jörg D.; Larsson, Tomas A.; Simakov, Oleg; Chapman, Emma C.; Arendt, Detlev; Rotchell, Jeanette M.

Abstract

An important priority for any organism is to maintain internal cellular homeostasis including acidbase balance. Yet, the molecular level impacts of changing environmental conditions, such as low pH, remain uncharacterised. Herein, we isolate partial Na+/H+ exchangers (NHE), carbonic anhydrase (CA), and calmodulin (CaM) genes from a polychaete, Platynereis dumerilii and investigate their relative expression in acidified seawater conditions. mRNA expression of NHE was significantly down-regulated after 1 h and up-regulated after 7 days under low pH treatment (pH 7.8), indicating changes in acid-base transport. Furthermore, the localisation of NHE expression was also altered. A trend of down regulation in CA after 1 h was also observed, suggesting a shift in the CO2 and HCO3- balance. No change in CaM expression was detected after 7 days exposure to acidified seawater. This study provides insight into the molecular level changes taking place following exposure to acidified seawater in a non-calcifying, ubiquitous, organism.

Citation

Wäge, J., Hardege, J. D., Larsson, T. A., Simakov, O., Chapman, E. C., Arendt, D., & Rotchell, J. M. (2015). Effects of low seawater pH on the marine polychaete Platynereis dumerilii. Marine pollution bulletin, 95(1), 166-172. https://doi.org/10.1016/j.marpolbul.2015.04.027

• Journal Article Type: Article
• Acceptance Date: Apr 11, 2015
• Online Publication Date: Apr 22, 2015
• Publication Date: Jun 15, 2015
• Deposit Date: Apr 14, 2015
• Publicly Available Date: Oct 27, 2022
• Journal: Marine pollution bulletin
• Print ISSN: 0025-326X
• Electronic ISSN: 1879-3363
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 95
• Issue: 1
• Pages: 166-172
• DOI: https://doi.org/10.1016/j.marpolbul.2015.04.027
• Keywords: Acid-base regulation, Low pH, Gene expression, Polychaeta, Platynereis dumerilii
• Public URL: https://hull-repository.worktribe.com/output/372383
• Publisher URL: http://www.sciencedirect.com/science/article/pii/S0025326X15002295
• Additional Information: This article is maintained by: Elsevier; Article Title: Effects of low seawater pH on the marine polychaete Platynereis dumerilii; Journal Title: Marine Pollution Bulletin; CrossRef DOI link to publisher maintained version: http://dx.doi.org/10.1016/j.marpolbul.2015.04.027; Content Type: article; Copyright: Copyright © 2015 Elsevier Ltd. All rights reserved.