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Unravelling the Occurrence of Mediator-Blood Protein Interactions via the Redox Catalysis of the Physiological Gasotransmitter Hydrogen Sulfide

Ward, Haydn J; Ward, Rhys A; Lawrence, Nathan S.; Wadhawan, Jay D.

Authors

Haydn J Ward

Rhys A Ward

Nathan S. Lawrence



Abstract

Ferrocenemethanol is employed as an aqueous, homogeneous redox mediator for hydrogen sulfide. The reaction is seen to follow an EC’ mechanism over the range 6≤pH≤9, with bisulfide reacting four times more rapidly than hydrogen sulfide. In the presence of 10 vol.% hæmolysed blood, greater concentrations of sulfide (as H2S or as HS−) are required to achieve the same degree of redox catalysis, compared with the absence of the blood proteins, at both pH 7 and pH 9. It is suggested that this phenomenon derives from a ferrocenemethanol/blood protein interaction, which is first titrated by the sulfide species. This can give rise to a titration-based electroanalytical assay for sulfide in blood, whilst being important for blood-based electrochemical bioassays involving hydrophilic ferrocene derivatives.

Citation

Ward, H. J., Ward, R. A., Lawrence, N. S., & Wadhawan, J. D. (2021). Unravelling the Occurrence of Mediator-Blood Protein Interactions via the Redox Catalysis of the Physiological Gasotransmitter Hydrogen Sulfide. ChemistrySelect, 6(37), 10059-10062. https://doi.org/10.1002/slct.202102974

Journal Article Type Article
Acceptance Date Sep 14, 2021
Online Publication Date Oct 5, 2021
Publication Date Oct 6, 2021
Deposit Date Sep 14, 2021
Publicly Available Date Oct 6, 2022
Journal ChemistrySelect
Electronic ISSN 2365-6549
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 6
Issue 37
Pages 10059-10062
DOI https://doi.org/10.1002/slct.202102974
Keywords Biosensors; Cyclic voltammetry; Electrochemistry; Ferrocene derivatives; Redox catalysis; Sensors
Public URL https://hull-repository.worktribe.com/output/3839553