Unravelling the Occurrence of Mediator-Blood Protein Interactions via the Redox Catalysis of the Physiological Gasotransmitter Hydrogen Sulfide

Ward, Haydn J; Ward, Rhys A; Lawrence, Nathan S.; Wadhawan, Jay D.

Abstract

Ferrocenemethanol is employed as an aqueous, homogeneous redox mediator for hydrogen sulfide. The reaction is seen to follow an EC’ mechanism over the range 6≤pH≤9, with bisulfide reacting four times more rapidly than hydrogen sulfide. In the presence of 10 vol.% hæmolysed blood, greater concentrations of sulfide (as H2S or as HS−) are required to achieve the same degree of redox catalysis, compared with the absence of the blood proteins, at both pH 7 and pH 9. It is suggested that this phenomenon derives from a ferrocenemethanol/blood protein interaction, which is first titrated by the sulfide species. This can give rise to a titration-based electroanalytical assay for sulfide in blood, whilst being important for blood-based electrochemical bioassays involving hydrophilic ferrocene derivatives.

Citation

Ward, H. J., Ward, R. A., Lawrence, N. S., & Wadhawan, J. D. (2021). Unravelling the Occurrence of Mediator-Blood Protein Interactions via the Redox Catalysis of the Physiological Gasotransmitter Hydrogen Sulfide. ChemistrySelect, 6(37), 10059-10062. https://doi.org/10.1002/slct.202102974

• Journal Article Type: Article
• Acceptance Date: Sep 14, 2021
• Online Publication Date: Oct 5, 2021
• Publication Date: Oct 6, 2021
• Deposit Date: Sep 14, 2021
• Publicly Available Date: Oct 6, 2022
• Journal: ChemistrySelect
• Electronic ISSN: 2365-6549
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 6
• Issue: 37
• Pages: 10059-10062
• DOI: https://doi.org/10.1002/slct.202102974
• Keywords: Biosensors; Cyclic voltammetry; Electrochemistry; Ferrocene derivatives; Redox catalysis; Sensors
• Public URL: https://hull-repository.worktribe.com/output/3839553