Skip to main content

Research Repository

Advanced Search

Porphyrin-nanosensor conjugates. New tools for the measurement of intracellular response to reactive oxygen species

Josefsen, Leanne B.; Aylott, Jonathan W.; Beeby, Andrew; Warburton, Philip; Boyle, John P.; Peers, Chris; Boyle, Ross W.

Authors

Leanne B. Josefsen

Jonathan W. Aylott

Andrew Beeby

Philip Warburton

John P. Boyle

Chris Peers



Abstract

Reactive oxygen species (ROS) have for some time been implicated in the onset and progression of medical conditions including cancer, ageing, heart disease and Alzheimer's disease. Recently, it has been postulated that ROS play a much more subtle role in intracellular signalling mechanisms as second messengers. Given the importance of these species in influencing cellular processes, it is surprising that tools for studying intracellular levels of ROS are extremely limited and devices for studying the cells' response to internally generated ROS are virtually non-existent. In order to study the response of cells to intracellular ROS we have designed a nano-scale device that can both generate ROS and simultaneously monitor the cells' reaction as a function of changes in the important signalling ion, calcium. Here we report the synthesis, characterisation, and calibration of a new ROS nano-probe and demonstrate its ability to detect cellular response to elevated levels of intracellular ROS.

Citation

Josefsen, L. B., Aylott, J. W., Beeby, A., Warburton, P., Boyle, J. P., Peers, C., & Boyle, R. W. (2010). Porphyrin-nanosensor conjugates. New tools for the measurement of intracellular response to reactive oxygen species. Photochemical & photobiological sciences : an international journal, 9(6), 801-811. https://doi.org/10.1039/c0pp00004c

Journal Article Type Article
Acceptance Date Apr 12, 2010
Online Publication Date May 12, 2010
Publication Date Jun 7, 2010
Publicly Available Date
Journal PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES
Print ISSN 1474-905X
Electronic ISSN 1474-9092
Publisher Royal Society of Chemistry
Peer Reviewed Peer Reviewed
Volume 9
Issue 6
Pages 801-811
DOI https://doi.org/10.1039/c0pp00004c
Keywords Physical and Theoretical Chemistry
Public URL https://hull-repository.worktribe.com/output/391385
Publisher URL http://pubs.rsc.org/en/Content/ArticleLanding/2010/PP/c0pp00004c#!divAbstract