University of Hull logo

Controlled intracellular generation of reactive oxygen species in human mesenchymal stem cells using porphyrin conjugated nanoparticles

Lavado, Andrea S.; Aylott, Jonathan W.; Chauhan, Veeren M.; Zen, Amer Alhaj; Giuntini, Francesca; Jones, D. Rhodri E.; Boyle, Ross W.; Beeby, Andrew; Chan, Weng C.; Aylott, Jonathan W.

Authors

Andrea S. Lavado

Jonathan W. Aylott

Veeren M. Chauhan

Amer Alhaj Zen

Francesca Giuntini

D. Rhodri E. Jones

Professor Ross Boyle R.W.Boyle@hull.ac.uk
Professor of Biological Chemistry/ Module Coordinator for Biological Macromolecules/ Biological Safety and Genetic Modifications Committee Member

Andrew Beeby

Weng C. Chan

Jonathan W. Aylott

Abstract

Nanoparticles capable of generating controlled amounts of intracellular reactive oxygen species (ROS), that advance the study of oxidative stress and cellular communication, were synthesized by functionalizing polyacrylamide nanoparticles with zinc(II) porphyrin photosensitisers. Controlled ROS production was demonstrated in human mesenchymal stem cells (hMSCs) through (1) production of nanoparticles functionalized with varying percentages of Zn(II) porphyrin and (2) modulating the number of doses of excitation light to internalized nanoparticles. hMSCs challenged with nanoparticles functionalized with increasing percentages of Zn(II) porphyrin and high numbers of irradiations of excitation light were found to generate greater amounts of ROS. A novel dye, which is transformed into fluorescent 7-hydroxy-4-trifluoromethyl-coumarin in the presence of hydrogen peroxide, provided an indirect indicator for cumulative ROS production. The mitochondrial membrane potential was monitored to investigate the destructive effect of increased intracellular ROS production. Flow cytometric analysis of nanoparticle treated hMSCs suggested irradiation with excitation light signalled controlled apoptotic cell death, rather than uncontrolled necrotic cell death. Increased intracellular ROS production did not induce phenotypic changes in hMSC subcultures.

Journal Article Type Article
Publication Date Aug 5, 2015
Journal Nanoscale
Print ISSN 2040-3364
Electronic ISSN 2040-3372
Publisher Royal Society of Chemistry
Peer Reviewed Peer Reviewed
Volume 7
Issue 34
Pages 14525-14531
Institution Citation Lavado, A. S., Chauhan, V. M., Zen, A. A., Giuntini, F., Jones, D. R. E., Boyle, R. W., …Aylott, J. W. (2015). Controlled intracellular generation of reactive oxygen species in human mesenchymal stem cells using porphyrin conjugated nanoparticles. Nanoscale, 7(34), 14525-14531. https://doi.org/10.1039/c5nr00795j
DOI https://doi.org/10.1039/c5nr00795j
Keywords Porphyrin conjugated nanoparticles, Intracellular reactive oxygen species, Human mesenchymal stem cells , ROS, hMSCs
Publisher URL http://pubs.rsc.org/en/Content/ArticleLanding/2015/NR/C5NR00795J#!divAbstract
Additional Information Authors' accepted manuscript of article published in: Nanoscale, 2015, v.7, issue 34.

Files

Article.pdf (477 Kb)
PDF

Copyright Statement
This work is licensed under a Creative Commons Attribution 3.0 Unported License.



You might also like


Downloadable Citations