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Conjugatable water-soluble Pt(ii) and Pd(ii) porphyrin complexes: Novel nano- and molecular probes for optical oxygen tension measurement in tissue engineering

Giuntini, F.; Chauhan, V. M.; Aylott, J. W.; Rosser, G. A.; Athanasiadis, A.; Beeby, A.; MacRobert, A. J.; Brown, R. A.; Boyle, R. W.

Authors

F. Giuntini

V. M. Chauhan

J. W. Aylott

G. A. Rosser

A. Athanasiadis

A. Beeby

A. J. MacRobert

R. A. Brown



Abstract

Measurement of oxygen tension in compressed collagen sheets was performed using matrix-embedded optical oxygen sensors based on platinum(II) and palladium(II) porphyrins supported on polyacrylamide nanoparticles. Bespoke, fully water-soluble, mono-functionalised Pt(II) and Pd(II) porphyrin complexes designed for conjugation under mild conditions were obtained using microwave-assisted metallation. The new sensors display a linear response (1/τ vs. O₂) to varying oxygen tension over a biologically relevant range (7.0 × 10⁻⁴ to 2.7 × 10⁻¹ mM) in aqueous solutions; a behaviour that is maintained following conjugation to polyacrylamide nanoparticles, and following embedding of the nanosensors in compressed collagen sheets, paving the way to innovative approaches for real-time resolution of oxygen gradients throughout 3D matrices useful for tissue regeneration.

Publication Date Apr 30, 2014
Journal Photochemical & photobiological sciences
Print ISSN 1474-905X
Electronic ISSN 1474-9092
Publisher Royal Society of Chemistry
Peer Reviewed Peer Reviewed
Volume 13
Issue 7
Pages 1039-1051
Institution Citation Giuntini, F., Chauhan, V. M., Aylott, J. W., Rosser, G. A., Athanasiadis, A., Beeby, A., …Boyle, R. W. (2014). Conjugatable water-soluble Pt(ii) and Pd(ii) porphyrin complexes: Novel nano- and molecular probes for optical oxygen tension measurement in tissue engineering. Photochemical & photobiological sciences : an international journal, 13(7), 1039-1051. https://doi.org/10.1039/c4pp00026a
DOI https://doi.org/10.1039/c4pp00026a
Keywords Oxygen tension, Porphyrin complexes , Tissue engineering
Publisher URL http://pubs.rsc.org/en/Content/ArticleLanding/2014/PP/c4pp00026a#!divAbstract
Copyright Statement ©2015 University of Hull
Additional Information Copy of article first published in: Photochemical & photobiological sciences, 2014, v.13, issue 7.

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