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Conjugatable water-soluble Pt(ii) and Pd(ii) porphyrin complexes: Novel nano- and molecular probes for optical oxygen tension measurement in tissue engineering

Giuntini, F.; Chauhan, V. M.; Aylott, J. W.; Rosser, G. A.; Athanasiadis, A.; Beeby, A.; MacRobert, A. J.; Brown, R. A.; Boyle, R. W.

Authors

F. Giuntini

V. M. Chauhan

J. W. Aylott

G. A. Rosser

A. Athanasiadis

A. Beeby

A. J. MacRobert

R. A. Brown

Professor Ross Boyle R.W.Boyle@hull.ac.uk
Professor of Biological Chemistry/ Module Coordinator for Biological Macromolecules/ Biological Safety and Genetic Modifications Committee Member

Abstract

Measurement of oxygen tension in compressed collagen sheets was performed using matrix-embedded optical oxygen sensors based on platinum(II) and palladium(II) porphyrins supported on polyacrylamide nanoparticles. Bespoke, fully water-soluble, mono-functionalised Pt(II) and Pd(II) porphyrin complexes designed for conjugation under mild conditions were obtained using microwave-assisted metallation. The new sensors display a linear response (1/τ vs. O₂) to varying oxygen tension over a biologically relevant range (7.0 × 10⁻⁴ to 2.7 × 10⁻¹ mM) in aqueous solutions; a behaviour that is maintained following conjugation to polyacrylamide nanoparticles, and following embedding of the nanosensors in compressed collagen sheets, paving the way to innovative approaches for real-time resolution of oxygen gradients throughout 3D matrices useful for tissue regeneration.

Publication Date Apr 30, 2014
Journal Photochemical & photobiological sciences
Print ISSN 1474-905X
Electronic ISSN 1474-9092
Publisher Royal Society of Chemistry
Peer Reviewed Peer Reviewed
Volume 13
Issue 7
Pages 1039-1051
Institution Citation Giuntini, F., Chauhan, V. M., Aylott, J. W., Rosser, G. A., Athanasiadis, A., Beeby, A., …Boyle, R. W. (2014). Conjugatable water-soluble Pt(ii) and Pd(ii) porphyrin complexes: Novel nano- and molecular probes for optical oxygen tension measurement in tissue engineering. Photochemical & photobiological sciences : an international journal, 13(7), 1039-1051. https://doi.org/10.1039/c4pp00026a
DOI https://doi.org/10.1039/c4pp00026a
Keywords Oxygen tension, Porphyrin complexes , Tissue engineering
Publisher URL http://pubs.rsc.org/en/Content/ArticleLanding/2014/PP/c4pp00026a#!divAbstract
Copyright Statement ©2015 University of Hull
Additional Information Copy of article first published in: Photochemical & photobiological sciences, 2014, v.13, issue 7.

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