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Identification, release and olfactory detection of bile salts in the intestinal fluid of the Senegalese sole (Solea senegalensis)

Hardege, Joerg D.; Canário, Adelino V M; Velez, Zelia; Canario, Adelino V. M.; Velez, Zélia; Hubbard, Peter C.; Welham, Kevin; Hardege, Jorg; Barata, Eduardo N.; Canário, Adelino V. M.

Authors

Joerg D. Hardege

Adelino V M Canário

Zelia Velez

Adelino V. M. Canario

Zélia Velez

Peter C. Hubbard

Kevin Welham K.J.Welham@hull.ac.uk

Eduardo N. Barata

Adelino V. M. Canário



Abstract

Olfactory sensitivity to bile salts is wide-spread in teleosts; however, which bile salts are released in sufficient quantities to be detected is unclear. The current study identified bile salts in the intestinal and bile fluids of Solea senegalensis by mass spectrometry-liquid chromatography and assessed their olfactory potency by the electro-olfactogram. The main bile salts identified in the bile were taurocholic acid (342 mM) and taurolithocholic acid (271 mM) plus a third, unidentified, bile salt of 532.3 Da. These three were also present in the intestinal fluid (taurocholic acid, 4.13 mM; taurolithocholic acid, 0.4 mM). In sole-conditioned water, only taurocholic acid (0.31 μM) was released in sufficient quantities to be measured (release rate: 24 nmol kg -1 min -1 ). Sole had high olfactory sensitivity to taurocholic acid but not to taurolithocholic acid. Furthermore, olfactory sensitivity was higher in the upper (right) olfactory epithelium than the lower (left). These two bile acids contribute about 40% of the olfactory potency of intestinal fluid and account for the difference in potency at the two epithelia. Taurocholic acid (but not taurolithocholic acid), and possibly other types of bile acid not tested, could be used as chemical signals and the upper olfactory epithelium is specialised for their detection. © Springer-Verlag 2009.

Journal Article Type Article
Publication Date 2009-07
Journal JOURNAL OF COMPARATIVE PHYSIOLOGY A-NEUROETHOLOGY SENSORY NEURAL AND BEHAVI
Print ISSN 0340-7594
Electronic ISSN 1432-1351
Publisher Springer Verlag
Peer Reviewed Peer Reviewed
Volume 195
Issue 7
Pages 691-698
APA6 Citation Velez, Z., Hubbard, P. C., Welham, K., Hardege, J., Barata, E. N., & Canário, A. V. M. (2009). Identification, release and olfactory detection of bile salts in the intestinal fluid of the Senegalese sole (Solea senegalensis). Journal of comparative physiology. A, Sensory, neural, and behavioral physiology, 195(7), (691-698). doi:10.1007/s00359-009-0444-5. ISSN 0340-7594
DOI https://doi.org/10.1007/s00359-009-0444-5
Keywords Animal Science and Zoology; Physiology; Ecology, Evolution, Behavior and Systematics; Behavioral Neuroscience
Publisher URL https://link.springer.com/article/10.1007%2Fs00359-009-0444-5
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