Development of a monolith based immobilized lipase micro-reactor for biocatalytic reactions in a biphasic mobile system

He, Ping; Greenway, Gillian; Haswell, Stephen J.

Abstract

This paper reports a simple method for producing macroporous silica-monoliths with controllable porosity that can be used for the immobilization of lipases to generate an active and stable micro-reactor for biocatalysis. A range of commercially available lipases has been examined using the hydrolysis reactions of 4-nitrophenyl butyrate in water-decane media. The kinetic studies performed have identified that a similar value for k(cat) is obtained for the immobilized Candida antarctica lipase A (0.13 min(-1)) and the free lipase in solution (0.12 min(-1)) whilst the immobilized apparent Michaelis constant K-m (3.1 mM) is 12 times lower than the free lipase in solution (38 mM). A 96% conversion was obtained for the immobilized C. antarctica lipase A compared to only 23% conversion for the free lipase. The significant higher conversions obtained with the immobilized lipases were mainly attributed to the formation of a favourable biphasic system in the continuous flowing micro-reactor system, where a significant increase in the interfacial activation occurred. The immobilized C. antarctica lipase A on the monolith also exhibited improved stability, showing 64% conversion at 80 degrees C and 70% conversion after continuous running for 480 h, compared to 40 and 20% conversions under the same temperature and reaction time for the free lipase.

Citation

He, P., Greenway, G., & Haswell, S. J. (2010). Development of a monolith based immobilized lipase micro-reactor for biocatalytic reactions in a biphasic mobile system. Process Biochemistry, 45(4), 593-597. https://doi.org/10.1016/j.procbio.2009.12.008

• Journal Article Type: Article
• Acceptance Date: Dec 10, 2009
• Online Publication Date: Dec 16, 2009
• Publication Date: 2010-04
• Journal: PROCESS BIOCHEMISTRY
• Print ISSN: 1359-5113
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 45
• Issue: 4
• Pages: 593-597
• DOI: https://doi.org/10.1016/j.procbio.2009.12.008
• Keywords: Micro-reactor; Immobilized lipase; Monolith; Biphasic system; Hydrolysis reaction; Enzyme kinetics
• Public URL: https://hull-repository.worktribe.com/output/391661
• Publisher URL: https://www.sciencedirect.com/science/article/pii/S1359511309003900?via%3Dihub