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The influence of exogenous tissue factor on the regulators of proliferation and apoptosis in endothelial cells

Pradier, Amandine; Ettelaie, Camille

Authors

Amandine Pradier

Camille Ettelaie C.Ettelaie@hull.ac.uk



Abstract

Background: The exposure of tissue factor (TF) at the site of injury or trauma is a rapid process that leads to the initiation of blood coagulation as well as homeostatic processes giving rise to vascular repair. Aims and Methods: By exposing human endothelial cells to combinations of exogenous TF and factor VIIa (FVIIa) in serum-free medium, the influence of TF concentrations on cellular proliferation and apoptosis was investigated. Results: Lower concentrations of TF resulted in increased cellular proliferation as well as upregulation of cyclin D1, downregulation of p21 and p27 and induction of tube formation in vitro. Conversely, incubation with higher concentrations of TF resulted in the activation of caspase-3, expression of p53 and Bax, translocation of p53 into the nucleus and induction of DNA fragmentation. Incubation of the cells with TF/FVIIa led to a lower proliferation rate with additional upregulation in p27. Conclusions: TF seems to have a bifunctional role in determining the fate of endothelial cells, depending on the concentration and the interactions of this protein. The release of TF in the locality of the injured tissue makes this protein an ideal factor for ascertaining the level of injury and determining the fate of the cells. Copyright © 2007 S. Karger AG.

Journal Article Type Article
Publication Date Dec 1, 2007
Journal JOURNAL OF VASCULAR RESEARCH
Print ISSN 1018-1172
Electronic ISSN 1423-0135
Publisher Karger Publishers
Peer Reviewed Peer Reviewed
Volume 45
Issue 1
Pages 19-32
APA6 Citation Pradier, A., & Ettelaie, C. (2007). The influence of exogenous tissue factor on the regulators of proliferation and apoptosis in endothelial cells. Journal of vascular research, 45(1), 19-32. doi:10.1159/000109074
DOI https://doi.org/10.1159/000109074
Keywords Physiology; Cardiology and Cardiovascular Medicine
Publisher URL http://content.karger.com/produktedb/produkte.asp?DOI=000109074&typ=pdf
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