Serum IL10 and circulating CD4+CD25high regulatory T cell numbers as predictors of clinical outcome and survival in patients with head and neck squamous cell carcinoma

Alhamarneh, Osama; Agada, Frank; Madden, Leigh; Stafford, Nicholas; Greenman, John

doi:10.1002/hed.21464

Serum IL10 and circulating CD4+CD25high regulatory T cell numbers as predictors of clinical outcome and survival in patients with head and neck squamous cell carcinoma

Alhamarneh, Osama; Agada, Frank; Madden, Leigh; Stafford, Nicholas; Greenman, John

Authors

Osama Alhamarneh

Frank Agada

Leigh Madden

Nicholas Stafford

Professor John Greenman J.Greenman@hull.ac.uk
Professor of Tumour Immunology

Abstract

Background. Patients with head and neck squamous cell carcinoma (HNSCC) commonly have an imbalance in T helper (Th)1/Th2-type cytokines and elevated levels of CD4 + CD25 high regulatory T cells (Treg). Here, we investigated the association of circulating interleukin (IL)10, IL12, and Treg-cells with clinical outcome in patients with HNSCC. Methods. Serum cytokine levels were determined by enzyme-linked immunosorbent assay (ELISA) in patients' pretreatment (n = 107) and 4 to 6 weeks posttreatment (n = 43), and in nontumor controls (n = 40). Treg-cell levels were determined by flow cytometry. Results. IL10 detectability was significantly higher in patients than controls (p =.001). Pretreatment IL10 levels in all anatomical subsites, except the oral cavity, were significantly elevated in stages III/IV, N+ patients, and in T3/4-tumors (p =.005,.037, and.001, respectively). The detectability of IL10 significantly correlated with poorer survival after a maximum follow-up of 36 months. Treg-cell levels did not correlate with any clinical parameters. Conclusion. IL10 is a potential independent factor in predicting a poor clinical outcome in newly presenting tumors of laryngeal and pharyngeal origin. The role of circulating Treg-cells as predictors of clinical outcome requires further investigation. Copyright © 2009 Wiley Periodicals, Inc.

Citation

Alhamarneh, O., Agada, F., Madden, L., Stafford, N., & Greenman, J. (2011). Serum IL10 and circulating CD4+CD25high regulatory T cell numbers as predictors of clinical outcome and survival in patients with head and neck squamous cell carcinoma. Head & neck, 33(3), 415-423. https://doi.org/10.1002/hed.21464

Journal Article Type	Article
Acceptance Date	Mar 30, 2010
Online Publication Date	Jul 19, 2010
Publication Date	Mar 1, 2011
Journal	HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK
Print ISSN	1043-3074
Publisher	Wiley
Peer Reviewed	Peer Reviewed
Volume	33
Issue	3
Pages	415-423
DOI	https://doi.org/10.1002/hed.21464
Keywords	Otorhinolaryngology
Public URL	https://hull-repository.worktribe.com/output/400122