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In Silico Genomic and Metabolic Atlas of Limosilactobacillus reuteri DSM 20016: An Insight into Human Health

Smythe, Paisleigh; Efthimiou, Georgios


Paisleigh Smythe


Probiotics are bacterial strains that are known to provide host health benefits. Limosilacto-bacillus reuteri is a well-documented lactic acid bacterium that has been cultured from numerous human sites. The strain investigated was L. reuteri DSM 20016, which has been found to produce useful metabolites. The strain was explored using genomic and proteomic tools, manual searches, and databases, including KEGG, STRING, BLAST Sequence Similarity Search, and UniProt. This study located over 200 key genes that were involved in human health benefit pathways. L. reuteri DSM 20016 has metabolic pathways to produce acetate, propionate, and lactate, and there is evidence of a pathway for butanoate production through a FASII mechanism. The bacterium produces histamine through the hdc operon, which may be able to suppress proinflammatory TNF, and the bacterium also has the ability to synthesize folate and riboflavin, although whether they are secreted is yet to be explored. The strain can bind to human Caco2 cells through srtA, mapA/cnb, msrB, and fbpA and can compete against enteric bacteria using reuterin, which is an antimicrobial that induces oxidative stress. The atlas could be used for designing metabolic engineering approaches to improve beneficial metabolite biosynthesis and better probiotic-based cures.


Smythe, P., & Efthimiou, G. (2022). In Silico Genomic and Metabolic Atlas of Limosilactobacillus reuteri DSM 20016: An Insight into Human Health. Microorganisms, 10(7), Article 1341.

Journal Article Type Article
Acceptance Date Jun 30, 2022
Online Publication Date Jul 2, 2022
Publication Date Jul 2, 2022
Deposit Date Jul 4, 2022
Publicly Available Date Oct 27, 2022
Journal Microorganisms
Electronic ISSN 2076-2607
Publisher MDPI
Peer Reviewed Peer Reviewed
Volume 10
Issue 7
Article Number 1341
Keywords Probiotic; Health benefits; Adhesion; Genome; Metabolome; Reuterin; Competition; Lactic acid bacteria; Limosilactobacillus reuteri
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Copyright Statement
Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
This article is an open access article distributed under the terms and
conditions of the Creative Commons Attribution (CC BY) license (

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