Multi-omic analysis of two common p53 mutations: Proteins regulated by mutated p53 as potential targets for immunotherapy

Vadakekolathu, Jayakumar; Boocock, David J.; Pandey, Kirti; Guinn, Barbara Ann; Legrand, Antoine; Miles, Amanda K.; Coveney, Clare; Ayala, Rochelle; Purcell, Anthony W.; McArdle, Stephanie E.

Abstract

The p53 protein is mutated in more than 50% of human cancers. Mutated p53 proteins not only lose their normal function but often acquire novel oncogenic functions, a phenomenon termed mutant p53 gain-of-function. Mutant p53 has been shown to affect the transcription of a range of genes, as well as protein–protein interactions with transcription factors and other effectors; however, no one has intensively investigated and identified these proteins, or their MHC presented epitopes, from the viewpoint of their ability to act as targets for immunotherapeutic interventions. We investigated the molecular changes that occurred after the TP53 null osteosarcoma cells, SaOS-2, were transfected with one of two conformational p53-mutants, either R175H or R273H. We then examined the phenotypic and functional changes using macroscopic observations, proliferation, gene expression and proteomics alongside immunopeptidome profiling of peptide antigen presentation in the context of major histocompatibility complex (MHC) class I molecules. We identified several candidate proteins in both TP53 mutant cell lines with differential expression when compared to the TP53 null vector control, SaOS-V. Quantitative SWATH proteomics combined with immune-peptidome analysis of the class-I eluted peptides identified several epitopes presented on pMHC and in silico analysis shortlisted which antigens were expressed in a range of cancerous but not adjacent healthy tissues. Out of all the candidates, KLC1 and TOP2A showed high levels of expression in every tumor type examined. From these proteins, three A2 and four pan HLA-A epitopes were identified in both R175H and R273H from TOP2A. We have now provided a short list of future immunotherapy targets for the treatment of cancers harboring mutated TP53.

Citation

Vadakekolathu, J., Boocock, D. J., Pandey, K., Guinn, B. A., Legrand, A., Miles, A. K., …McArdle, S. E. (2022). Multi-omic analysis of two common p53 mutations: Proteins regulated by mutated p53 as potential targets for immunotherapy. Cancers, 14(16), Article 3975. https://doi.org/10.3390/cancers14163975

• Journal Article Type: Article
• Acceptance Date: Aug 13, 2022
• Online Publication Date: Aug 17, 2022
• Publication Date: Aug 2, 2022
• Deposit Date: Dec 18, 2022
• Publicly Available Date: Jan 3, 2023
• Journal: Cancers
• Electronic ISSN: 2072-6694
• Publisher: MDPI
• Peer Reviewed: Peer Reviewed
• Volume: 14
• Issue: 16
• Article Number: 3975
• DOI: https://doi.org/10.3390/cancers14163975
• Keywords: p53, SaOS-2, R273H, R175H, mass spectrometry, MHC peptides, immunotherapy, conformational mutants.
• Public URL: https://hull-repository.worktribe.com/output/4051365
• Publisher URL: https://www.mdpi.com/journal/cancers