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Inflammatory stimuli up-regulate transient receptor potential vanilloid-1 expression in human bronchial fibroblasts

Sadofsky, Laura R.; Sadofsky, Laura; Ramachandran, Rithwik; Morice, Alyn; Crow, Christopher; Cowen, Michael; Compton, Steven J.; Morice, Alyn H.


Rithwik Ramachandran

Christopher Crow

Michael Cowen

Steven J. Compton


Lung fibroblasts are involved in interstitial lung disease, chronic asthma, and chronic obstructive pulmonary disease (COPD). The expanded fibroblast population in airway disease leads to airway remodeling and contributes to the inflammatory process seen in these diseases. The cation channel transient receptor potential vanilloid-1 (TRPV1) is activated by noxious stimuli, including capsaicin, protons, and high temperatures and is thought to have a role in inflammation. Although TRPV1 expression is primarily reported to be neuronal, some extraneuronal expression has been reported. The authors therefore sought to determine whether human primary bronchial fibroblasts (HPBFs) express TRPV1 and whether inflammatory mediators can induce TRPV1 expression. The authors show that fibroblasts are predominantly TRPV1 negative; however, following stimulation with 3 common inflammatory mediators, tumor necrosis factor α (TNF-α), lipopolysaccharide (LPS), and interleukin-1α (IL-1α), TRPV1 mRNA was observed at 24 and 48 hours post treatment with all 3 mediators. Using Western blotting an increase in TRPV1 expression with all 3 inflammatory mediators was detected with significant increases seen at 72 hours post LPS and IL-1α treatment. In stark contrast to the untreated fibroblasts, significant calcium signaling in response to capsaicin and resiniferatoxin in HPBFs treated for 24 and 48 hours with TNF-α, LPS, or IL-1α was also observed. These results indicate that TRPV1 can be expressed on bronchial fibroblasts in situations where an underlying inflammatory stimulus exists, as is the case in airway diseases such as asthma and COPD.


Sadofsky, L. R., Ramachandran, R., Crow, C., Cowen, M., Compton, S. J., & Morice, A. H. (2012). Inflammatory stimuli up-regulate transient receptor potential vanilloid-1 expression in human bronchial fibroblasts. Experimental Lung Research, 38(2), 75-81.

Journal Article Type Article
Acceptance Date Nov 21, 2011
Online Publication Date Jan 13, 2012
Publication Date Feb 2, 2012
Journal Experimental lung research
Print ISSN 0190-2148
Electronic ISSN 1521-0499
Publisher Taylor and Francis
Peer Reviewed Peer Reviewed
Volume 38
Issue 2
Pages 75-81
Keywords Clinical Biochemistry; Molecular Biology; Pulmonary and Respiratory Medicine
Public URL
PMID 22242698