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Distinguishing Properties of Cells From the Myocardial Sleeves of the Pulmonary Veins: A Comparison of Normal and Abnormal Pacemakers

Jones, Sandra; Jones, Sandra A.; Yamamoto, Mitsuru; Tellez, James O.; Billeter, Rudi; Boyett, Mark R.; Honjo, Haruo; Lancaster, Matthew K.

Authors

Mitsuru Yamamoto

James O. Tellez

Rudi Billeter

Mark R. Boyett

Haruo Honjo

Matthew K. Lancaster



Abstract

BACKGROUND: A common source of arrhythmogenic spontaneous activity instigating atrial fibrillation is the myocardial tissue, or sleeves, at the base of the pulmonary veins. This study compared the properties of cells from the myocardial sleeves of the pulmonary veins (PV(m)) with cells from the normal cardiac pacemaker (the sinoatrial node) and regions of the atria. Our objective was to identify key features of these cells that predispose them to becoming the focus of cardiac arrhythmias. METHODS AND RESULTS: Single cells were isolated from samples of rabbit PV(m), central and peripheral sinoatrial node, crista terminalis, and left and right atria. Detailed morphology of cells was assessed and intracellular calcium concentrations measured with the use of Fluo-3. Cells from the PV(m) were smaller than atrial cells and showed large elevations in diastolic calcium during activation at physiological rates, a feature the PV(m) cells shared with cells from the sinoatrial node. Unstimulated spontaneous activity was observed in a minority of cells from the PV(m), but numerous cells from this region showed spontaneous activity for a brief period immediately subsequent to stimulation at physiological rates. This was not observed in atrial cells. Assessment of calcium removal pathways showed sarcolemmal calcium extrusion in cells from the PV(m) to have a high reliance on "slow" extrusion pathways to maintain intracellular calcium homeostasis because of a low expression of sodium-calcium exchanger. CONCLUSIONS: We conclude that cells from the PV(m) share some features with cells from the sinoatrial node but also have distinctly unique features that predispose them to the development of spontaneous activity.

Journal Article Type Article
Publication Date Apr 1, 2008
Journal CIRCULATION-ARRHYTHMIA AND ELECTROPHYSIOLOGY
Print ISSN 1941-3149
Electronic ISSN 1941-3084
Publisher American Heart Association
Peer Reviewed Peer Reviewed
Volume 1
Issue 1
Pages 39-48
APA6 Citation Jones, S. A., Yamamoto, M., Tellez, J. O., Billeter, R., Boyett, M. R., Honjo, H., & Lancaster, M. K. (2008). Distinguishing Properties of Cells From the Myocardial Sleeves of the Pulmonary Veins: A Comparison of Normal and Abnormal Pacemakers. Circulation. Arrhythmia and electrophysiology, 1(1), 39-48. doi:10.1161/CIRCEP.107.748467
DOI https://doi.org/10.1161/CIRCEP.107.748467
Keywords arrhythmia; pacemakers; pulmonary vein; sinoatrial node; intracellular calcium; atria
Publisher URL http://circep.ahajournals.org/content/1/1/39
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