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Auto-fluorescent PAMAM-based dendritic molecules and their potential application in pharmaceutical sciences

El-Betany, Alaa M.M.; Kamoun, Elbadawy A.; James, Craig; Jangher, Abdulhakim; Aljayyoussi, Ghaith; Griffiths, Peter; McKeown, Neil B.; Gumbleton, Mark

Authors

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Dr Alaa Elbetany A.M.Elbetany@hull.ac.uk
Lecturer in Organic and Materials Chemistry

Elbadawy A. Kamoun

Craig James

Abdulhakim Jangher

Ghaith Aljayyoussi

Peter Griffiths

Neil B. McKeown

Mark Gumbleton



Abstract

The epithelial permeation of water-soluble fluorescent PAMAM dendrons based on 7H-benz[de] benzimidazo [2,1-a] isoquinoline-7-one as a fluorescent core across epithelial cell models MDCK I and MDCK II has been quantified. Hydrodynamic radii have been derived from self-diffusion coefficients obtained via pulsed-gradient spin-echo Nuclear Magnetic Resonance (PGSE-NMR). Results indicate that these dendritic molecules are molecularly disperse, non-aggregating, and only slightly larger than their parent homologues. MDCK I permeability studies across epithelial barriers show that these dendritic molecules are biocompatible with the chosen epithelial in-vitro model and can permeate across MDCK cell monolayers. Permeability is demonstrated to be a property of dendritic size and cell barrier restrictiveness indicating that paracellular mechanisms play the predominant role in the transport of these molecules.

Citation

El-Betany, A. M., Kamoun, E. A., James, C., Jangher, A., Aljayyoussi, G., Griffiths, P., …Gumbleton, M. (2020). Auto-fluorescent PAMAM-based dendritic molecules and their potential application in pharmaceutical sciences. International Journal of Pharmaceutics, 579, Article 119187. https://doi.org/10.1016/j.ijpharm.2020.119187

Journal Article Type Article
Acceptance Date Feb 26, 2020
Online Publication Date Mar 2, 2020
Publication Date Apr 15, 2020
Deposit Date Feb 25, 2023
Journal International Journal of Pharmaceutics
Print ISSN 0378-5173
Electronic ISSN 1873-3476
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 579
Article Number 119187
DOI https://doi.org/10.1016/j.ijpharm.2020.119187
Keywords PAMAM dendrons; Water-soluble fluorescent dendrons; Self-diffusion coefficient; Hydrodynamic radii; Permeability; Imaging and visualisation
Public URL https://hull-repository.worktribe.com/output/4225011
Related Public URLs https://orca.cardiff.ac.uk/id/eprint/130325/