Auto-fluorescent PAMAM-based dendritic molecules and their potential application in pharmaceutical sciences

El-Betany, Alaa M.M.; Kamoun, Elbadawy A.; James, Craig; Jangher, Abdulhakim; Aljayyoussi, Ghaith; Griffiths, Peter; McKeown, Neil B.; Gumbleton, Mark

Abstract

The epithelial permeation of water-soluble fluorescent PAMAM dendrons based on 7H-benz[de] benzimidazo [2,1-a] isoquinoline-7-one as a fluorescent core across epithelial cell models MDCK I and MDCK II has been quantified. Hydrodynamic radii have been derived from self-diffusion coefficients obtained via pulsed-gradient spin-echo Nuclear Magnetic Resonance (PGSE-NMR). Results indicate that these dendritic molecules are molecularly disperse, non-aggregating, and only slightly larger than their parent homologues. MDCK I permeability studies across epithelial barriers show that these dendritic molecules are biocompatible with the chosen epithelial in-vitro model and can permeate across MDCK cell monolayers. Permeability is demonstrated to be a property of dendritic size and cell barrier restrictiveness indicating that paracellular mechanisms play the predominant role in the transport of these molecules.

Citation

El-Betany, A. M., Kamoun, E. A., James, C., Jangher, A., Aljayyoussi, G., Griffiths, P., McKeown, N. B., & Gumbleton, M. (2020). Auto-fluorescent PAMAM-based dendritic molecules and their potential application in pharmaceutical sciences. International Journal of Pharmaceutics, 579, Article 119187. https://doi.org/10.1016/j.ijpharm.2020.119187

• Journal Article Type: Article
• Acceptance Date: Feb 26, 2020
• Online Publication Date: Mar 2, 2020
• Publication Date: Apr 15, 2020
• Deposit Date: Feb 25, 2023
• Journal: International Journal of Pharmaceutics
• Print ISSN: 0378-5173
• Electronic ISSN: 1873-3476
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 579
• Article Number: 119187
• DOI: https://doi.org/10.1016/j.ijpharm.2020.119187
• Keywords: PAMAM dendrons; Water-soluble fluorescent dendrons; Self-diffusion coefficient; Hydrodynamic radii; Permeability; Imaging and visualisation
• Public URL: https://hull-repository.worktribe.com/output/4225011
• Related Public URLs: https://orca.cardiff.ac.uk/id/eprint/130325/