Influence of photoinitiator concentration and irradiation time on the crosslinking performance of visible-light activated pullulan-HEMA hydrogels

Kamoun, Elbadawy A.; El-Betany, Alaa; Menzel, Henning; Chen, Xin

Abstract

In-situ forming hydrogels were prepared from pullulan-HEMA copolymer using three-component visible-light system composed of camphorquinone carboxylic acid-folic acid-iodonium salt. The relevance of double bond conversion and crosslinking density of hydrogels with the photoinitiator concentration and irradiation time were estimated by FT-IR analysis and swelling calculation using Flory-Rehner theory, respectively. The results revealed that the crosslinking density and degree of conversion of hydrogels were improved by photoinitiator concentration increasing until certain extend, then they decreased due to a primary radicals termination reaction occurred. The shortest irradiation time of 10 s was essential to obtain acceptable hydrogels for further characterizations. For the probability use of hydrogels as scaffold was investigated in vitro by measuring of the indirect cytotoxicity assay by MTT-assay using human bone Sarcoma cell as a reference cell lines. The majority of seeded SW1353 cells maintained a live with an accepted viability of ~85–92% over a four days culture period with irradiation of hydrogel 10 s, while cell viability has improved to ~95–98% with prolonging the irradiation time of hydrogel to 60 s. The current photoinitiating system is a proper system for in-situ crosslinking the activated-light biomaterials for bone regeneration, dental, or tissue engineering applications.

Citation

Kamoun, E. A., El-Betany, A., Menzel, H., & Chen, X. (2018). Influence of photoinitiator concentration and irradiation time on the crosslinking performance of visible-light activated pullulan-HEMA hydrogels. International journal of biological macromolecules, 120, 1884-1892. https://doi.org/10.1016/j.ijbiomac.2018.10.011

• Journal Article Type: Article
• Acceptance Date: Oct 1, 2018
• Online Publication Date: Oct 2, 2018
• Publication Date: Dec 1, 2018
• Deposit Date: Feb 25, 2023
• Journal: International Journal of Biological Macromolecules
• Print ISSN: 0141-8130
• Electronic ISSN: 1879-0003
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 120
• Pages: 1884-1892
• DOI: https://doi.org/10.1016/j.ijbiomac.2018.10.011
• Keywords: Pullulan-HEMA; Carboxylated camphorquinone; Folic acid
• Public URL: https://hull-repository.worktribe.com/output/4225022
• Related Public URLs: https://orca.cardiff.ac.uk/id/eprint/116662/