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Evaluation of heart tissue viability under redox-magnetohydrodynamics conditions: Toward fine-tuning flow in biological microfluidics applications

Cheah, Lih Tyng; Fritsch, Ingrid; Haswell, Stephen J.; Greenman, John

Authors

Lih Tyng Cheah

Ingrid Fritsch

Stephen J. Haswell



Abstract

A microfluidic system containing a chamber for heart tissue biopsies, perfused with Krebs–Henseleit buffer containing glucose and antibiotic (KHGB) using peristaltic pumps and continuously stimulated, was used to evaluate tissue viability under redox-magnetohydrodynamics (redox- MHD) conditions. Redox-MHD possesses unique capabilities to control fluid flow using ionic current from oxidation and reduction processes at electrodes in a magnetic field, making it attractive to fine-tune fluid flow around tissues for ‘‘tissueon- a-chip’’ applications. The manuscript describes a parallel setup to study two tissue samples simultaneously, and 6-min static incubation with Triton X100. Tissue viability was subsequently determined by assaying perfusate for lactate dehydrogenase (LDH) activity, where LDH serves as an injury marker. Incubation with KHGB containing 5mM hexaammineruthenium(III) (ruhex) redox species with and without a pair of NdFeB magnets ( the chamber) exhibited no additional tissue insult. MHD fluid flow, viewed by tracking microbeads with microscopy, occurred only when the magnet was present and stimulating electrodes were activated. Pulsating MHD flow with a frequency similar to the stimulating waveform was superimposed over thermal convection (from a hotplate) for Triton-KHGB, but fluid speed was up to twice as fast for ruhex-Triton-KHGB. A large transient ionic current, achieved when switching on the stimulating electrodes, generates MHDperturbations visible over varying peristaltic flow. The well-controlled flow methodology of redox-MHD is applicable to any tissue type, being useful in various drug uptake and toxicity studies, and can be combined equally with on- or off-device analysis modalities.0.39 T, placed parallel to

Journal Article Type Article
Publication Date Jul 31, 2012
Journal BIOTECHNOLOGY AND BIOENGINEERING
Print ISSN 0006-3592
Electronic ISSN 1097-0290
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 109
Issue 7
Pages 1827-1834
APA6 Citation Cheah, L. T., Fritsch, I., Haswell, S. J., & Greenman, J. (2012). Evaluation of heart tissue viability under redox-magnetohydrodynamics conditions: Toward fine-tuning flow in biological microfluidics applications. Biotechnology and bioengineering, 109(7), 1827-1834. doi:10.1002/bit.24426
DOI https://doi.org/10.1002/bit.24426
Keywords heart tissue; microfluidics; redox-magnetohydrodynamics; lactate dehydrogenase; Triton X100; hexaammineruthenium(III) chloride
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