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Microfluidic perfusion system for maintaining viable heart tissue with real-time electrochemical monitoring of reactive oxygen species

Dou, Yue-Hua; Seymour, Anne-Marie L.; Cheah, Lih Tyng; Cheah, Lih-Tyng; Dou, Yue Hua; Seymour, Anne Marie L.; Dyer, Charlotte E.; Haswell, Stephen J.; Wadhawan, Jay D.; Greenman, John

Authors

Yue-Hua Dou

Anne-Marie L. Seymour

Lih Tyng Cheah

Lih-Tyng Cheah

Yue Hua Dou

Anne Marie L. Seymour

Charlotte E. Dyer

Stephen J. Haswell



Abstract

A microfluidic device has been developed to maintain viable heart tissue samples in a biomimetic microenvironment. This device allows rat or human heart tissue to be studied under pseudo in vivo conditions. Effluent levels of lactate dehydrogenase and hydrogen peroxide were used as markers of damaged tissue in combination with in situ electrochemical measurement of the release of reactive oxygen species (ROS). The parameters for perfusion were optimized to maintain biopsies of rat right ventricular or human right atrial tissue viable for up to 5 and 3.5 hours, respectively. Electrochemical assessment of the oxidation current of total ROS, employing cyclic voltammetry, gave results in real-time that were in good agreement to biochemical assessment using conventional, off-chip, commercial assays. This proof-of-principle, integrated microfluidic device, may be exploited in providing a platform technology for future cardiac research, offering an alternative approach for investigating heart pathophysiology and facilitating the development of new therapeutic strategies.

Journal Article Type Article
Publication Date Oct 21, 2010
Journal Lab on a chip
Print ISSN 1473-0197
Electronic ISSN 1473-0189
Publisher Royal Society of Chemistry
Peer Reviewed Peer Reviewed
Volume 10
Issue 20
Pages 2720-2726
APA6 Citation Cheah, L., Dou, Y. H., Seymour, A. M. L., Dyer, C. E., Haswell, S. J., Wadhawan, J. D., & Greenman, J. (2010). Microfluidic perfusion system for maintaining viable heart tissue with real-time electrochemical monitoring of reactive oxygen species. Lab on a chip, 10(20), 2720-2726 . https://doi.org/10.1039/c004910g
DOI https://doi.org/10.1039/c004910g
Keywords Intracellular calcium; Single macrophage; Cyclic voltammetry; Hydrogen-peroxide; Cardiac myocyte; Cell culture; Release device; Chemiluminescence
Publisher URL http://pubs.rsc.org/en/Content/ArticleLanding/2010/LC/c004910g#!divAbstract
Additional Information Authors' accepted manuscript of article: Cheah, Lih-Tyng and Dou, Yue-Hua and Seymour, Anne-Marie L. and Dyer, Charlotte E. and Haswell, Stephen J. and Wadhawan, Jay D. and Greenman, John. Microfluidic perfusion system for maintaining viable heart tissue with real-time electrochemical monitoring of reactive oxygen species. Lab Chip, 2010, volume 10, issue 20

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