Microfluidic perfusion system for maintaining viable heart tissue with real-time electrochemical monitoring of reactive oxygen species

Cheah, Lih-Tyng; Dou, Yue Hua; Seymour, Anne Marie L.; Dyer, Charlotte E.; Haswell, Stephen J.; Wadhawan, Jay D.; Greenman, John

Abstract

A microfluidic device has been developed to maintain viable heart tissue samples in a biomimetic microenvironment. This device allows rat or human heart tissue to be studied under pseudo in vivo conditions. Effluent levels of lactate dehydrogenase and hydrogen peroxide were used as markers of damaged tissue in combination with in situ electrochemical measurement of the release of reactive oxygen species (ROS). The parameters for perfusion were optimized to maintain biopsies of rat right ventricular or human right atrial tissue viable for up to 5 and 3.5 hours, respectively. Electrochemical assessment of the oxidation current of total ROS, employing cyclic voltammetry, gave results in real-time that were in good agreement to biochemical assessment using conventional, off-chip, commercial assays. This proof-of-principle, integrated microfluidic device, may be exploited in providing a platform technology for future cardiac research, offering an alternative approach for investigating heart pathophysiology and facilitating the development of new therapeutic strategies.

Citation

Cheah, L., Dou, Y. H., Seymour, A. M. L., Dyer, C. E., Haswell, S. J., Wadhawan, J. D., & Greenman, J. (2010). Microfluidic perfusion system for maintaining viable heart tissue with real-time electrochemical monitoring of reactive oxygen species. Lab on a chip, 10(20), 2720-2726. https://doi.org/10.1039/c004910g

• Journal Article Type: Article
• Acceptance Date: Jul 26, 2010
• Online Publication Date: Aug 19, 2010
• Publication Date: Oct 21, 2010
• Deposit Date: Nov 13, 2014
• Publicly Available Date: Nov 13, 2014
• Journal: Lab on a chip
• Print ISSN: 1473-0197
• Electronic ISSN: 1473-0189
• Publisher: Royal Society of Chemistry
• Peer Reviewed: Peer Reviewed
• Volume: 10
• Issue: 20
• Pages: 2720-2726
• DOI: https://doi.org/10.1039/c004910g
• Keywords: Intracellular calcium; Single macrophage; Cyclic voltammetry; Hydrogen-peroxide; Cardiac myocyte; Cell culture; Release device; Chemiluminescence
• Public URL: https://hull-repository.worktribe.com/output/462001
• Publisher URL: http://pubs.rsc.org/en/Content/ArticleLanding/2010/LC/c004910g#!divAbstract
• Additional Information: Authors' accepted manuscript of article: Cheah, Lih-Tyng and Dou, Yue-Hua and Seymour, Anne-Marie L. and Dyer, Charlotte E. and Haswell, Stephen J. and Wadhawan, Jay D. and Greenman, John. Microfluidic perfusion system for maintaining viable heart tissue with real-time electrochemical monitoring of reactive oxygen species. Lab Chip, 2010, volume 10, issue 20