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The Arp2/3 activator WASH regulates α5β1-integrin-mediated invasive migration

Zech, Tobias; Calaminus, Simon; Caswell, Patrick; Spence, Heather J.; Carnell, Michael; Insall, Robert H.; Norman, Jim; Machesky, Laura M.


Tobias Zech

Simon Calaminus

Patrick Caswell

Heather J. Spence

Michael Carnell

Robert H. Insall

Jim Norman

Laura M. Machesky


The actin cytoskeleton provides scaffolding and physical force to effect fundamental processes such as motility, cytokinesis and vesicle trafficking. The Arp2/3 complex nucleates actin structures and contributes to endocytic vesicle invagination and trafficking away from the plasma membrane. Internalisation and directed recycling of integrins are major driving forces for invasive cell motility and potentially for cancer metastasis. Here, we describe a direct requirement for WASH and Arp2/3-mediated actin polymerisation on the endosomal membrane system for α5β1 integrin recycling. WASH regulates the trafficking of endosomal α5β1 integrin to the plasma membrane and is fundamental for integrin-driven cell morphology changes and integrin-mediated cancer cell invasion. Thus, we implicate WASH and Arp2/3-driven actin nucleation in receptor recycling leading to invasive motility.


Zech, T., Calaminus, S., Caswell, P., Spence, H. J., Carnell, M., Insall, R. H., …Machesky, L. M. (2012). The Arp2/3 activator WASH regulates α5β1-integrin-mediated invasive migration. Journal of cell science, 124(22), 3753-3759.

Journal Article Type Article
Acceptance Date Nov 23, 2012
Online Publication Date Nov 23, 2011
Publication Date Nov 23, 2012
Print ISSN 0021-9533
Electronic ISSN 1477-9137
Publisher The Company of Biologists
Peer Reviewed Peer Reviewed
Volume 124
Issue 22
Pages 3753-3759
Keywords Arp2/3 complex, invasion, actin assembly, integrin recycling, membrane trafficking
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