A standard, single dose of inhaled terbutaline attenuates hyperpnea-induced bronchoconstriction and mast cell activation in athletes

Simpson, A. J.; Bood, J. R.; Anderson, S. D.; Romer, L. M.; Dahlén, B.; Dahlén, S. E.; Kippelen, P.

Abstract

Release of bronchoactive mediators from mast cells during exercise hyperpnea is a key factor in the pathophysiology of exercise-induced bronchoconstriction (EIB). Our aim was to investigate the effect of a standard, single dose of an inhaled β 2 -adrenoceptor agonist on mast cell activation in response to dry air hyperpnea in athletes with EIB. Twenty-seven athletes with EIB completed a randomized, doubleblind, placebo-controlled, crossover study. Terbutaline (0.5 mg) or placebo was inhaled 15 min prior to 8 min of eucapnic voluntary hyperpnea (EVH) with dry air. Pre- and postbronchial challenge, urine samples were analyzed by enzyme immunoassay for 11β-prostaglandin F 2α (11β-PGF 2α ). The maximum fall in forced expiratory volume in 1 s of 14 (12-20)% (median and interquartile range) following placebo was attenuated to 7 (5-9)% with the administration of terbutaline (P < 0.001). EVH caused a significant increase in 11- PGF 2α from 41 (27-57) ng/mmol creatinine at baseline to 58 (43-72) ng/mmol creatinine at its peak post-EVH following placebo (P = 0.002). The rise in 11β-PGF 2α was inhibited with administration of terbutaline: 39 (28-44) ng/mmol creatinine at baseline vs. 40 (33-58) ng/mmol creatinine at its peak post-EVH (P = 0.118). These data provide novel in vivo evidence of mast cell stabilization following inhalation of a standard dose of terbutaline prior to bronchial provocation with EVH in athletes with EIB.

Citation

Simpson, A. J., Bood, J. R., Anderson, S. D., Romer, L. M., Dahlén, B., Dahlén, S. E., & Kippelen, P. (2016). A standard, single dose of inhaled terbutaline attenuates hyperpnea-induced bronchoconstriction and mast cell activation in athletes. Journal of Applied Physiology, 120(9), 1011-1017. https://doi.org/10.1152/japplphysiol.00700.2015

• Acceptance Date: Jan 28, 2016
• Online Publication Date: Feb 4, 2016
• Publication Date: May 1, 2016
• Deposit Date: Nov 23, 2017
• Publicly Available Date: Dec 7, 2017
• Journal: Journal of Applied Physiology
• Print ISSN: 8750-7587
• Publisher: American Physiological Society
• Peer Reviewed: Peer Reviewed
• Volume: 120
• Issue: 9
• Pages: 1011-1017
• DOI: https://doi.org/10.1152/japplphysiol.00700.2015
• Keywords: Inhaled β2-agonist; Exercise-induced bronchoconstriction; Eucapnic voluntary hyperpnea; Prostaglandin D2
• Public URL: https://hull-repository.worktribe.com/output/429634
• Publisher URL: http://jap.physiology.org/content/120/9/1011
• Contract Date: Dec 7, 2017