Ines Hahn
Tau, XMAP215/Msps and Eb1 co-operate interdependently to regulate microtubule polymerisation and bundle formation in axons
Hahn, Ines; Voelzmann, Andre; Parkin, Jill; Fülle, Judith B.; Slater, Paula G.; Lowery, Laura Anne; Sanchez-Soriano, Natalia; Prokop, Andreas
Authors
Dr Andre Voelzmann A.Voelzmann@hull.ac.uk
Lecturer in Cell Biology & Biotechnology
Jill Parkin
Judith B. Fülle
Paula G. Slater
Laura Anne Lowery
Natalia Sanchez-Soriano
Andreas Prokop
Abstract
The formation and maintenance of microtubules requires their polymerisation, but little is known about how this polymerisation is regulated in cells. Focussing on the essential microtubule bundles in axons of Drosophila and Xenopus neurons, we show that the plus-end scaffold Eb1, the polymerase XMAP215/Msps and the lattice-binder Tau co-operate interdependently to promote microtubule polymerisation and bundle organisation during axon development and maintenance. Eb1 and XMAP215/Msps promote each other's localisation at polymerising microtubule plus-ends. Tau outcompetes Eb1-binding along microtubule lattices, thus preventing depletion of Eb1 tip pools. The three factors genetically interact and show shared mutant phenotypes: reductions in axon growth, comet sizes, comet numbers and comet velocities, as well as prominent deterioration of parallel microtubule bundles into disorganised curled conformations. This microtubule curling is caused by Eb1 plus-end depletion which impairs spectraplakin-mediated guidance of extending microtubules into parallel bundles. Our demonstration that Eb1, XMAP215/Msps and Tau co-operate during the regulation of microtubule polymerisation and bundle organisation, offers new conceptual explanations for developmental and degenerative axon pathologies.
Citation
Hahn, I., Voelzmann, A., Parkin, J., Fülle, J. B., Slater, P. G., Lowery, L. A., Sanchez-Soriano, N., & Prokop, A. (2021). Tau, XMAP215/Msps and Eb1 co-operate interdependently to regulate microtubule polymerisation and bundle formation in axons. PLoS Genetics, 17(7), Article e1009647. https://doi.org/10.1371/journal.pgen.1009647
Journal Article Type | Article |
---|---|
Acceptance Date | Jun 7, 2021 |
Online Publication Date | Jul 6, 2021 |
Publication Date | Jul 6, 2021 |
Deposit Date | Feb 8, 2024 |
Publicly Available Date | Feb 14, 2024 |
Journal | PLoS Genetics |
Print ISSN | 1553-7390 |
Electronic ISSN | 1553-7404 |
Publisher | Public Library of Science |
Peer Reviewed | Peer Reviewed |
Volume | 17 |
Issue | 7 |
Article Number | e1009647 |
DOI | https://doi.org/10.1371/journal.pgen.1009647 |
Public URL | https://hull-repository.worktribe.com/output/4371859 |
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Copyright Statement
Copyright: © 2021 Hahn et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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