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Plastin 1 widens stereocilia by transforming actin filament packing from hexagonal to liquid

Krey, Jocelyn F.; Krystofiak, Evan S.; Dumont, Rachel A.; Vijayakumar, Sarath; Choi, Dongseok; Rivero, Francisco; Kachar, Bechara; Jones, Sherri M.; Barr-Gillespie, Peter G.

Authors

Jocelyn F. Krey

Evan S. Krystofiak

Rachel A. Dumont

Sarath Vijayakumar

Dongseok Choi

Bechara Kachar

Sherri M. Jones

Peter G. Barr-Gillespie



Abstract

With their essential role in inner-ear function, stereocilia of sensory hair cells demonstrate the importance of cellular actin protrusions. Actin packing in stereocilia is mediated by crosslinkers of the plastin, fascin, and espin families. While mice lacking ESPN (espin) have no vestibular or auditory function, we found that mice that either lacked PLS1 (plastin 1) or had nonfunctional FSCN2 (fascin 2) had reduced inner-ear function, with double-mutant mice most strongly affected. Targeted mass spectrometry indicated that PLS1 was the most abundant crosslinker in vestibular stereocilia, and the second-most-abundant protein overall; ESPN only accounted for ~15% of the total crosslinkers in bundles. Mouse utricle stereocilia lacking PLS1 were shorter and thinner than wild-type stereocilia. Surprisingly, while wild-type stereocilia had random liquid packing of their actin filaments, stereocilia lacking PLS1 had orderly hexagonal packing. While all three crosslinkers are required for stereocilia structure and function, PLS1 biases actin towards liquid packing, which allows stereocilia to grow to a greater diameter.

Citation

Krey, J. F., Krystofiak, E. S., Dumont, R. A., Vijayakumar, S., Choi, D., Rivero, F., Kachar, B., Jones, S. M., & Barr-Gillespie, P. G. (2016). Plastin 1 widens stereocilia by transforming actin filament packing from hexagonal to liquid. Journal of Cell Biology, 215(4), 467-482. https://doi.org/10.1083/jcb.201606036

Journal Article Type Article
Acceptance Date Oct 11, 2016
Online Publication Date Nov 3, 2016
Publication Date Nov 21, 2016
Deposit Date Oct 6, 2016
Publicly Available Date Nov 3, 2016
Journal Journal of cell biology
Print ISSN 0021-9525
Publisher Rockefeller University Press
Peer Reviewed Peer Reviewed
Volume 215
Issue 4
Pages 467-482
DOI https://doi.org/10.1083/jcb.201606036
Keywords Cell Biology
Public URL https://hull-repository.worktribe.com/output/443843
Publisher URL http://jcb.rupress.org/content/early/2016/11/02/jcb.201606036
Additional Information Copy of article first published in: Journal of cell biology, 2016, v.214 issue 4.
Contract Date Oct 6, 2016

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Publisher Licence URL
https://creativecommons.org/licenses/by-nc-sa/3.0/

Copyright Statement
© 2016 Krey et al.
This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).






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