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Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches

Welsh, Joshua A.; Goberdhan, Deborah C.I.; O'Driscoll, Lorraine; Buzas, Edit I.; Blenkiron, Cherie; Bussolati, Benedetta; Cai, Houjian; Di Vizio, Dolores; Driedonks, Tom A.P.; Erdbrügger, Uta; Falcon-Perez, Juan M.; Fu, Qing Ling; Hill, Andrew F.; Lenassi, Metka; Lim, Sai Kiang; Mahoney, Mỹ G.; Mohanty, Sujata; Möller, Andreas; Nieuwland, Rienk; Ochiya, Takahiro; Sahoo, Susmita; Torrecilhas, Ana C.; Zheng, Lei; Zijlstra, Andries; Abuelreich, Sarah; Bagabas, Reem; Bergese, Paolo; Bridges, Esther M.; Brucale, Marco; Burger, Dylan; Carney, Randy P.; Cocucci, Emanuele; Crescitelli, Rossella; Hanser, Edveena; Harris, Adrian L.; Haughey, Norman J.; Hendrix, An; Ivanov, Alexander R.; Jovanovic-Talisman, Tijana; Kruh-Garcia, Nicole A.; Ku'ulei-Lyn Faustino, Vroniqa; Kyburz, Diego; Lässer, Cecilia; Lennon, Kathleen M.; Lötvall, Jan; Maddox, Adam L.; Martens-Uzunova, Elena S.; Mizenko, Rachel R.; Newman, Lauren A.; Ridolfi, Andrea; Rohde, Eva; Rojalin, Tatu; Rowland, Andrew; Saftics, Andras; San...

Authors

Joshua A. Welsh

Deborah C.I. Goberdhan

Lorraine O'Driscoll

Edit I. Buzas

Cherie Blenkiron

Benedetta Bussolati

Houjian Cai

Dolores Di Vizio

Tom A.P. Driedonks

Uta Erdbrügger

Juan M. Falcon-Perez

Qing Ling Fu

Andrew F. Hill

Metka Lenassi

Sai Kiang Lim

Mỹ G. Mahoney

Sujata Mohanty

Andreas Möller

Rienk Nieuwland

Takahiro Ochiya

Susmita Sahoo

Ana C. Torrecilhas

Lei Zheng

Andries Zijlstra

Sarah Abuelreich

Reem Bagabas

Paolo Bergese

Esther M. Bridges

Marco Brucale

Dylan Burger

Randy P. Carney

Emanuele Cocucci

Rossella Crescitelli

Edveena Hanser

Adrian L. Harris

Norman J. Haughey

An Hendrix

Alexander R. Ivanov

Tijana Jovanovic-Talisman

Nicole A. Kruh-Garcia

Vroniqa Ku'ulei-Lyn Faustino

Diego Kyburz

Cecilia Lässer

Kathleen M. Lennon

Jan Lötvall

Adam L. Maddox

Elena S. Martens-Uzunova

Rachel R. Mizenko

Lauren A. Newman

Andrea Ridolfi

Eva Rohde

Tatu Rojalin

Andrew Rowland

Andras Saftics

Ursula S. Sandau

Julie A. Saugstad

Faezeh Shekari

Simon Swift

Dmitry Ter-Ovanesyan

Juan P. Tosar

Zivile Useckaite

Francesco Valle

Zoltan Varga

Edwin van der Pol

Martijn J.C. van Herwijnen

Marca H.M. Wauben

Ann M. Wehman

Sarah Williams

Andrea Zendrini

Alan J. Zimmerman

Misev Consortium

Clotilde Théry

Kenneth W. Witwer



Contributors

Abstract

Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its 'Minimal Information for Studies of Extracellular Vesicles', which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly.

Citation

Welsh, J. A., Goberdhan, D. C., O'Driscoll, L., Buzas, E. I., Blenkiron, C., Bussolati, B., Cai, H., Di Vizio, D., Driedonks, T. A., Erdbrügger, U., Falcon-Perez, J. M., Fu, Q. L., Hill, A. F., Lenassi, M., Lim, S. K., Mahoney, M. G., Mohanty, S., Möller, A., Nieuwland, R., Ochiya, T., …Witwer, K. W. (2024). Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches. Journal of Extracellular Vesicles, 13(2), Article e12404. https://doi.org/10.1002/jev2.12404

Journal Article Type Article
Acceptance Date Dec 19, 2023
Online Publication Date Feb 7, 2024
Publication Date Feb 1, 2024
Deposit Date Mar 5, 2024
Publicly Available Date Mar 5, 2024
Journal Journal of extracellular vesicles
Print ISSN 2001-3078
Electronic ISSN 2001-3078
Publisher Taylor & Francis Open Access
Peer Reviewed Peer Reviewed
Volume 13
Issue 2
Article Number e12404
DOI https://doi.org/10.1002/jev2.12404
Keywords Ectosomes; Exosomes; Extracellular vesicles; Extracellular particles; Guidelines; Microparticles; Microvesicles; Minimal information requirements; MISEV; Reproducibility; Rigor; Standardisation
Public URL https://hull-repository.worktribe.com/output/4572297

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Publisher Licence URL
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Copyright Statement
© 2024 The Authors. Journal of Extracellular Vesicles published by Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.




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