Joshua A. Welsh
Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches
Welsh, Joshua A.; Goberdhan, Deborah C.I.; O'Driscoll, Lorraine; Buzas, Edit I.; Blenkiron, Cherie; Bussolati, Benedetta; Cai, Houjian; Di Vizio, Dolores; Driedonks, Tom A.P.; Erdbrügger, Uta; Falcon-Perez, Juan M.; Fu, Qing Ling; Hill, Andrew F.; Lenassi, Metka; Lim, Sai Kiang; Mahoney, Mỹ G.; Mohanty, Sujata; Möller, Andreas; Nieuwland, Rienk; Ochiya, Takahiro; Sahoo, Susmita; Torrecilhas, Ana C.; Zheng, Lei; Zijlstra, Andries; Abuelreich, Sarah; Bagabas, Reem; Bergese, Paolo; Bridges, Esther M.; Brucale, Marco; Burger, Dylan; Carney, Randy P.; Cocucci, Emanuele; Crescitelli, Rossella; Hanser, Edveena; Harris, Adrian L.; Haughey, Norman J.; Hendrix, An; Ivanov, Alexander R.; Jovanovic-Talisman, Tijana; Kruh-Garcia, Nicole A.; Ku'ulei-Lyn Faustino, Vroniqa; Kyburz, Diego; Lässer, Cecilia; Lennon, Kathleen M.; Lötvall, Jan; Maddox, Adam L.; Martens-Uzunova, Elena S.; Mizenko, Rachel R.; Newman, Lauren A.; Ridolfi, Andrea; Rohde, Eva; Rojalin, Tatu; Rowland, Andrew; Saftics, Andras; San...
Authors
Deborah C.I. Goberdhan
Lorraine O'Driscoll
Edit I. Buzas
Cherie Blenkiron
Benedetta Bussolati
Houjian Cai
Dolores Di Vizio
Tom A.P. Driedonks
Uta Erdbrügger
Juan M. Falcon-Perez
Qing Ling Fu
Andrew F. Hill
Metka Lenassi
Sai Kiang Lim
Mỹ G. Mahoney
Sujata Mohanty
Andreas Möller
Rienk Nieuwland
Takahiro Ochiya
Susmita Sahoo
Ana C. Torrecilhas
Lei Zheng
Andries Zijlstra
Sarah Abuelreich
Reem Bagabas
Paolo Bergese
Esther M. Bridges
Marco Brucale
Dylan Burger
Randy P. Carney
Emanuele Cocucci
Rossella Crescitelli
Edveena Hanser
Adrian L. Harris
Norman J. Haughey
An Hendrix
Alexander R. Ivanov
Tijana Jovanovic-Talisman
Nicole A. Kruh-Garcia
Vroniqa Ku'ulei-Lyn Faustino
Diego Kyburz
Cecilia Lässer
Kathleen M. Lennon
Jan Lötvall
Adam L. Maddox
Elena S. Martens-Uzunova
Rachel R. Mizenko
Lauren A. Newman
Andrea Ridolfi
Eva Rohde
Tatu Rojalin
Andrew Rowland
Andras Saftics
Ursula S. Sandau
Julie A. Saugstad
Faezeh Shekari
Simon Swift
Dmitry Ter-Ovanesyan
Juan P. Tosar
Zivile Useckaite
Francesco Valle
Zoltan Varga
Edwin van der Pol
Martijn J.C. van Herwijnen
Marca H.M. Wauben
Ann M. Wehman
Sarah Williams
Andrea Zendrini
Alan J. Zimmerman
Misev Consortium
Clotilde Théry
Kenneth W. Witwer
Contributors
Dr Camille Ettelaie C.Ettelaie@hull.ac.uk
Other
Abstract
Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its 'Minimal Information for Studies of Extracellular Vesicles', which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly.
Citation
Welsh, J. A., Goberdhan, D. C., O'Driscoll, L., Buzas, E. I., Blenkiron, C., Bussolati, B., Cai, H., Di Vizio, D., Driedonks, T. A., Erdbrügger, U., Falcon-Perez, J. M., Fu, Q. L., Hill, A. F., Lenassi, M., Lim, S. K., Mahoney, M. G., Mohanty, S., Möller, A., Nieuwland, R., Ochiya, T., …Witwer, K. W. (2024). Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches. Journal of Extracellular Vesicles, 13(2), Article e12404. https://doi.org/10.1002/jev2.12404
Journal Article Type | Article |
---|---|
Acceptance Date | Dec 19, 2023 |
Online Publication Date | Feb 7, 2024 |
Publication Date | Feb 1, 2024 |
Deposit Date | Mar 5, 2024 |
Publicly Available Date | Mar 5, 2024 |
Journal | Journal of extracellular vesicles |
Print ISSN | 2001-3078 |
Electronic ISSN | 2001-3078 |
Publisher | Taylor & Francis Open Access |
Peer Reviewed | Peer Reviewed |
Volume | 13 |
Issue | 2 |
Article Number | e12404 |
DOI | https://doi.org/10.1002/jev2.12404 |
Keywords | Ectosomes; Exosomes; Extracellular vesicles; Extracellular particles; Guidelines; Microparticles; Microvesicles; Minimal information requirements; MISEV; Reproducibility; Rigor; Standardisation |
Public URL | https://hull-repository.worktribe.com/output/4572297 |
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Copyright Statement
© 2024 The Authors. Journal of Extracellular Vesicles published by Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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