Plastin 1 binds to keratin and is required for terminal web assembly in the intestinal epithelium

Grimm-Gunter, Eva Maria S; Grimm-Guenter, Eva-Maria S.; Ferrary, Evelyne; Grimm-Günter, Eva-Maria S.; Hurbain, Ilse; Louvard, Daniel; Ramos, Sonia; Revenu, Celine; Rivero, Francisco; Robine, Sylvie; Smyth, Neil

Abstract

Plastin 1 (I-plastin, fimbrin) along with villin and espin is a prominent actin-bundling protein of the intestinal brush border microvilli. We demonstrate here that plastin 1 accumulates in the terminal web and interacts with keratin 19, possibly contributing to anchoring the rootlets to the keratin network. This prompted us to investigate the importance of plastin 1 in brush border assembly. Although in vivo neither villin nor espin is required for brush border structure, plastin 1-deficient mice have conspicuous ultrastructural alterations: microvilli are shorter and constricted at their base, and, strikingly, their core actin bundles lack true rootlets. The composition of the microvilli themselves is apparently normal, whereas that of the terminal web is profoundly altered. Although the plastin 1 knockout mice do not show any overt gross phenotype and present a normal intestinal microanatomy, the alterations result in increased fragility of the epithelium. This is seen as an increased sensitivity of the brush border to biochemical manipulations, decreased transepithelial resistance, and increased sensitivity to dextran sodium sulfate-induced colitis. Plastin 1 thus emerges as an important regulator of brush border morphology and stability through a novel role in the organization of the terminal web, possibly by connecting actin filaments to the underlying intermediate filament network.

Citation

Grimm-Gunter, E. M. S., Grimm-Guenter, E. S., Ferrary, E., Grimm-Günter, E. S., Hurbain, I., Louvard, D., …Smyth, N. (2009). Plastin 1 binds to keratin and is required for terminal web assembly in the intestinal epithelium. Molecular Biology of the Cell, 20(10), 2549-2562. https://doi.org/10.1091/mbc.e08-10-1030

• Acceptance Date: Mar 12, 2009
• Online Publication Date: Mar 25, 2009
• Publication Date: May 15, 2009
• Deposit Date: Nov 13, 2014
• Publicly Available Date: Nov 13, 2014
• Journal: Molecular biology of the cell
• Print ISSN: 1059-1524
• Publisher: American Society for Cell Biology
• Peer Reviewed: Peer Reviewed
• Volume: 20
• Issue: 10
• Pages: 2549-2562
• DOI: https://doi.org/10.1091/mbc.e08-10-1030
• Keywords: Cell Biology; Molecular Biology
• Public URL: https://hull-repository.worktribe.com/output/460233
• Publisher URL: http://www.molbiolcell.org/content/20/10/2549
• Additional Information: Copy of article: Grimm-Günter, E.M., C. Revenu, S. Ramos, I. Hurbain, N. Smyth, E. Ferrary, D. Louvard, S. Robine, and F. Rivero. 2009. Plastin 1 binds to keratin and is required for terminal web assembly in the intestinal epithelium. Mol. Biol. Cell. 20:2549–2562. doi:10.1091/mbc.E08-10-1030