Photothermal colloid antibodies for shape-selective recognition and killing of microorganisms

Borovička, Josef; Metheringham, William J.; Madden, Leigh A.; Walton, Christopher D.; Stoyanov, Simeon D.; Paunov, Vesselin N.

Abstract

We have developed a class of selective antimicrobial agents based on the recognition of the shape and size of the bacterial cells. These agents are anisotropic colloid particles fabricated as negative replicas of the target cells which involve templating of the cells with shells of inert material followed by their fragmentation. The cell shape recognition by such shell fragments is due to the increased area of surface contact between the cells and their matching shell fragments which resembles antibody-antigen interaction. We produced such "colloid antibodies" with photothermal mechanism for shape-selective killing of matching cells. This was achieved by the subsequent deposition of (i) gold nanoparticles (AuNPs) and (ii) silica shell over yeast cells, which were chosen as model pathogens. We demonstrated that fragments of these composite AuNP/silica shells act as "colloid antibodies" and can bind to yeast cells of the same shape and size and deliver AuNPs directly onto their surface. We showed that after laser irradiation, the localized heating around the AuNPs kills the microbial cells of matching shape. We confirmed the cell shape-specific killing by photothermal colloid antibodies in a mixture of two bacterial cultures of different cell shape and size. This approach opens a number of avenues for building powerful selective biocides based on combinations of colloid antibodies and cell-killing strategies which can be applied in new antibacterial therapies.

Citation

Borovička, J., Metheringham, W. J., Madden, L. A., Walton, C. D., Stoyanov, S. D., & Paunov, V. N. (2013). Photothermal colloid antibodies for shape-selective recognition and killing of microorganisms. Journal of the American Chemical Society, 135(14), 5282-5285. https://doi.org/10.1021/ja400781f

• Journal Article Type: Article
• Acceptance Date: Jan 29, 2013
• Online Publication Date: Mar 29, 2013
• Publication Date: Apr 10, 2013
• Deposit Date: Nov 13, 2014
• Publicly Available Date: Nov 13, 2014
• Journal: Journal of the American Chemical Society
• Print ISSN: 0002-7863
• Electronic ISSN: 1520-5126
• Publisher: American Chemical Society
• Peer Reviewed: Peer Reviewed
• Volume: 135
• Issue: 14
• Pages: 5282-5285
• DOI: https://doi.org/10.1021/ja400781f
• Keywords: Colloid and Surface Chemistry; Biochemistry; General Chemistry; Catalysis
• Public URL: https://hull-repository.worktribe.com/output/469300
• Additional Information: This document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal of the American Chemical Society, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see dx.doi.org/10.1021/ja400781f