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Effects of α-lipoic acid on mtDNA damage after isolated muscle contractions

Fogarty, Mark C.; Devito, Giuseppe; Hughes, Ciara M.; Burke, George; Brown, John C.; McClean, Conor; McEneny, Jane; Brown, David; Davison, Gareth W.; McClean, Conor


Mark C. Fogarty

Giuseppe Devito

Ciara M. Hughes

George Burke

John C. Brown

Conor McClean

Jane McEneny

David Brown

Gareth W. Davison

Conor McClean


Introduction: Although pharmacological antioxidants have previously been investigated for a prophylactic effect against exercise oxidative stress, it is not known if [alpha]-lipoic acid supplementation can protect against DNA damage after high-intensity isolated quadriceps exercise. This randomized controlled investigation was designed to test the hypothesis that 14 d of [alpha]-lipoic acid supplementation can attenuate exercise-induced oxidative stress. Methods: Twelve (n = 12) apparently healthy male participants (age = 28 ± 10 yr, stature = 177 ± 12 cm and body mass = 81 ± 15 kg) were randomly assigned to receive either a daily supplement of 1000 mg of [alpha]-lipoic acid (2 × 500-mg tablets) for 14 d (n = 6) or receive no supplement (n = 6) in a double-blinded experimental approach. Blood and muscle biopsy tissue samples were taken at rest and after the completion of 100 isolated and continuous maximal knee extension (minimum force = 200 N, speed of contraction = 60°·s-1). Results: Exercise increased mitochondrial 8-hydroxy-2-deoxyguanosine (8-OHdG) concentration in both groups (P < 0.05 vs rest) with a concomitant decrease in total antioxidant capacity (P < 0.05 vs rest). There was a marked increase in blood total antioxidant capacity after oral [alpha]-lipoic acid supplementation (P < 0.05 vs nonsupplemented), whereas DNA damage (Comet assay and 8-OHdG), lipid peroxidation, and hydrogen peroxide increased after exercise in the nonsupplemented group only (P < 0.05 vs supplemented). Exercise increased protein oxidation in both groups (P < 0.05 vs rest). Conclusions: These findings suggest that short-term [alpha]-lipoic acid supplementation can selectively protect DNA (but not in muscle mitochondria) and lipids against exercise-induced oxidative stress.


Fogarty, M. C., Devito, G., Hughes, C. M., Burke, G., Brown, J. C., McClean, C., …McClean, C. Effects of α-lipoic acid on mtDNA damage after isolated muscle contractions. Medicine and Science in Sports and Exercise, 45(8), 1469-1477. doi:10.1249/MSS.0b013e31828bf31e

Journal Article Type Article
Deposit Date Nov 13, 2014
Journal Medicine and science in sports and exercise
Print ISSN 0195-9131
Electronic ISSN 1530-0315
Publisher Lippincott, Williams & Wilkins
Peer Reviewed Peer Reviewed
Volume 45
Issue 8
Pages 1469-1477
Keywords Isolated exercise, a-lipoic acid prophylaxis, Mitochondrial DNA damage, Lipid peroxidation
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