Mark C. Fogarty
Acute and chronic watercress supplementation attenuates exercise-induced peripheral mononuclear cell DNA damage and lipid peroxidation
Fogarty, Mark C.; Hughes, Ciara M.; Burke, George; Brown, John C.; Davison, Gareth W.
Ciara M. Hughes
John C. Brown
Gareth W. Davison
Pharmacological antioxidant vitamins have previously been investigated for a prophylactic effect against exercise-induced oxidative stress. However, large doses are often required and may lead to a state of pro-oxidation and oxidative damage. Watercress contains an array of nutritional compounds such as β-carotene and α-tocopherol which may increase protection against exercise-induced oxidative stress. The present randomised controlled investigation was designed to test the hypothesis that acute (consumption 2h before exercise) and chronic (8 weeks consumption) watercress supplementation can attenuate exercise-induced oxidative stress. A total of ten apparently healthy male subjects (age 23 (sd 4) years, stature 179 (sd 10)cm and body mass 74 (sd 15)kg) were recruited to complete the 8-week chronic watercress intervention period (and then 8 weeks of control, with no ingestion) of the experiment before crossing over in order to compete the single-dose acute phase (with control, no ingestion). Blood samples were taken at baseline (pre-supplementation), at rest (pre-exercise) and following exercise. Each subject completed an incremental exercise test to volitional exhaustion following chronic and acute watercress supplementation or control. The main findings show an exercise-induced increase in DNA damage and lipid peroxidation over both acute and chronic control supplementation phases (P<0·05 v. supplementation), while acute and chronic watercress attenuated DNA damage and lipid peroxidation and decreased H2O2 accumulation following exhaustive exercise (P<0·05 v. control). A marked increase in the main lipid-soluble antioxidants (α-tocopherol, γ-tocopherol and xanthophyll) was observed following watercress supplementation (P<0·05 v. control) in both experimental phases. These findings suggest that short- and long-term watercress ingestion has potential antioxidant effects against exercise-induced DNA damage and lipid peroxidation.
Fogarty, M. C., Hughes, C. M., Burke, G., Brown, J. C., & Davison, G. W. (2013). Acute and chronic watercress supplementation attenuates exercise-induced peripheral mononuclear cell DNA damage and lipid peroxidation. British Journal of Nutrition, 109(2), 293-301. doi:10.1017/S0007114512000992
|Journal Article Type||Article|
|Acceptance Date||Feb 13, 2012|
|Online Publication Date||Apr 5, 2012|
|Publication Date||Jan 28, 2013|
|Deposit Date||Nov 13, 2014|
|Journal||The British journal of nutrition|
|Publisher||Cambridge University Press (CUP)|
|Peer Reviewed||Peer Reviewed|
|Keywords||Exhaustive exercise; Watercress prophylaxis; DNA damage; Reactive oxygen species|
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