Skip to main content

Research Repository

Advanced Search

Western diet impairs metabolic remodelling and contractile efficiency in cardiac hypertrophy

Akki, Ashwin; Seymour, Anne-Marie L.


Ashwin Akki

Anne-Marie L. Seymour


Metabolic remodelling in cardiac hypertrophy is underscored by a reduction in fatty acid (FA) oxidation. We tested whether this decline in FA oxidation in the presence of enhanced FA supply may predispose the hypertrophied myocardium to lipid accumulation, functional deterioration, and eventually heart failure. and results Left ventricular hypertrophy was induced surgically in Sprague-Dawley rats by inter-renal aortic constriction. Rats were fed a Western diet (WD, 45% kcal from lipids) or standard diet (SD, 12% kcal from fat) for 9 weeks post-surgery. Hearts were perfused in the isovolumic mode with a physiological mixture of substrates including 5 mM 1-C-13 glucose, 1 mM 3-C-13 lactate, and 0.3 mM U-C-13 palmitate, and cardiac function was monitored. Real-time PCR was used to determine transcript levels of peroxisome proliferator-activated receptor-alpha (PPAR alpha) and PPAR alpha-regulated metabolic enzymes. Palmitate oxidation and PPAR alpha-regulated gene expression were markedly reduced in the hypertrophied myocardium of rats fed SD. However, 9 weeks of WD normalized both palmitate oxidation and PPAR alpha-regulated gene expression but significantly increased glucose and lactate oxidation in the hypertrophied hearts. This was accompanied by cardiac triglyceride accumulation and a decline in ventricular function despite an increase in oxygen consumption. These results highlight that WD-induced dysregulation of FA metabolism has deleterious functional consequences in cardiac hypertrophy.


Akki, A., & Seymour, A. L. (2009). Western diet impairs metabolic remodelling and contractile efficiency in cardiac hypertrophy. Cardiovascular research, 81(3), 610-617. doi:10.1093/cvr/cvn316

Journal Article Type Article
Acceptance Date Nov 18, 2008
Online Publication Date Nov 21, 2008
Publication Date Feb 15, 2009
Deposit Date Nov 13, 2014
Journal Cardiovascular Research
Print ISSN 0008-6363
Electronic ISSN 1755-3245
Publisher Oxford University Press (OUP)
Peer Reviewed Peer Reviewed
Volume 81
Issue 3
Pages 610-617
Keywords Physiology (medical); Physiology; Cardiology and Cardiovascular Medicine
Public URL