Skip to main content

Research Repository

Advanced Search

Mobile magnetic particles as solid-supports for rapid surface-based bioanalysis in continuous flow

Peyman, Sally A.; Iles, Alexander; Pamme, Nicole


Sally A. Peyman

Alexander Iles

Nicole Pamme


An extremely versatile microfluidic device is demonstrated in which multi-step (bio) chemical procedures can be performed in continuous flow. The system operates by generating several co-laminar flow streams, which contain reagents for specific (bio) reactions across a rectangular reaction chamber. Functionalised magnetic microparticles are employed as mobile solid-supports and are pulled from one side of the reaction chamber to the other by use of an external magnetic field. As the particles traverse the co-laminar reagent streams, binding and washing steps are performed on their surface in one operation in continuous flow. The applicability of the platform was first demonstrated by performing a proof-of-principle binding assay between streptavidin coated magnetic particles and biotin in free solution with a limit of detection of 20 ng mL(-1) of free biotin. The system was then applied to a mouse IgG sandwich immunoassay as a first example of a process involving two binding steps and two washing steps, all performed within 60 s, a fraction of the time required for conventional testing.


Peyman, S. A., Iles, A., & Pamme, N. (2009). Mobile magnetic particles as solid-supports for rapid surface-based bioanalysis in continuous flow. Lab on a chip, 9(21), 3110-3117.

Journal Article Type Article
Acceptance Date Jul 31, 2009
Online Publication Date Aug 21, 2009
Publication Date 2009
Deposit Date Nov 13, 2014
Journal Lab On A Chip
Print ISSN 1473-0197
Electronic ISSN 1473-0189
Publisher Royal Society of Chemistry
Peer Reviewed Peer Reviewed
Volume 9
Issue 21
Pages 3110-3117
Keywords Biochemistry; Bioengineering; General Chemistry; Biomedical Engineering
Public URL
Publisher URL!divAbstract