Mobile magnetic particles as solid-supports for rapid surface-based bioanalysis in continuous flow

Peyman, Sally A.; Iles, Alexander; Pamme, Nicole

Abstract

An extremely versatile microfluidic device is demonstrated in which multi-step (bio) chemical procedures can be performed in continuous flow. The system operates by generating several co-laminar flow streams, which contain reagents for specific (bio) reactions across a rectangular reaction chamber. Functionalised magnetic microparticles are employed as mobile solid-supports and are pulled from one side of the reaction chamber to the other by use of an external magnetic field. As the particles traverse the co-laminar reagent streams, binding and washing steps are performed on their surface in one operation in continuous flow. The applicability of the platform was first demonstrated by performing a proof-of-principle binding assay between streptavidin coated magnetic particles and biotin in free solution with a limit of detection of 20 ng mL(-1) of free biotin. The system was then applied to a mouse IgG sandwich immunoassay as a first example of a process involving two binding steps and two washing steps, all performed within 60 s, a fraction of the time required for conventional testing.

Citation

Peyman, S. A., Iles, A., & Pamme, N. (2009). Mobile magnetic particles as solid-supports for rapid surface-based bioanalysis in continuous flow. Lab on a chip, 9(21), 3110-3117. https://doi.org/10.1039/b904724g

• Journal Article Type: Article
• Acceptance Date: Jul 31, 2009
• Online Publication Date: Aug 21, 2009
• Publication Date: 2009
• Deposit Date: Nov 13, 2014
• Journal: Lab On A Chip
• Print ISSN: 1473-0197
• Publisher: Royal Society of Chemistry
• Peer Reviewed: Peer Reviewed
• Volume: 9
• Issue: 21
• Pages: 3110-3117
• DOI: https://doi.org/10.1039/b904724g
• Keywords: Biochemistry; Bioengineering; General Chemistry; Biomedical Engineering
• Public URL: https://hull-repository.worktribe.com/output/472879
• Publisher URL: http://pubs.rsc.org/en/Content/ArticleLanding/2009/LC/b904724g#!divAbstract
• Contract Date: Nov 13, 2014