Silver Nanoparticle-Immobilized Schiff-Base Macrocycles as Nanozymes with Peroxidase Mimic Activity for Antibacterial Films

Wang, Kuiyuan; Redshaw, Carl; Zhao, Xuesong; Wang, Anheng; Chen, Kai; Qiao, Yuanting

Abstract

The development of nanozymes has emerged as a promising pathway for combating bacterial infections. In this work, Ag/Schiff base nanozymes are obtained through the in situ reduction method on [2 + 3] Schiff base macrocycles using eco-friendly reductant α-tocopherol. The silver prepared was found to be nanoscale by TEM, and Ag NPs grew and dispersed uniformly on the surface of the Schiff base macrocycle due to the abundance of the C=N double bonds. The peroxidase-like catalytic activity of the Ag/Schiff base was evaluated toward 3,3′,5,5′-tetramethylben-zidine (TMB) in the presence of H2O2, and the Km values of 0.350 and 0.090 mM confirm the strong affinity of Ag/Schiff base for H2O2 and TMB. Moreover, the nanozyme exhibited good antibacterial properties against Streptococcus sanguinis, Pseudomonas aeruginosa, and Staphylococcus aureus, and in vivo tests confirmed that the Ag/Schiff base had negligible biotoxicity. Benefiting from the good antimicrobial and biosafety properties, the nanozymes were explored to prepare antibacterial films, and the bacterial inhibition zones demonstrated that the introduction of the Ag/Schiff base could prevent the growth of bacteria.

Citation

Wang, K., Redshaw, C., Zhao, X., Wang, A., Chen, K., & Qiao, Y. (2024). Silver Nanoparticle-Immobilized Schiff-Base Macrocycles as Nanozymes with Peroxidase Mimic Activity for Antibacterial Films. ACS Applied Nano Materials, 7(14), 16274–16282. https://doi.org/10.1021/acsanm.4c02258

• Journal Article Type: Article
• Acceptance Date: Jun 25, 2024
• Online Publication Date: Jul 12, 2024
• Publication Date: Jul 26, 2024
• Deposit Date: Jul 13, 2024
• Publicly Available Date: Jul 13, 2025
• Journal: ACS Applied Nano Materials
• Print ISSN: 2574-0970
• Publisher: American Chemical Society
• Peer Reviewed: Peer Reviewed
• Volume: 7
• Issue: 14
• Pages: 16274–16282
• DOI: https://doi.org/10.1021/acsanm.4c02258
• Keywords: Bacteria, Composites, Macrocycles, Metal nanoparticles, Peptides and proteins
• Public URL: https://hull-repository.worktribe.com/output/4736478