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Target gene expression studies on Platynereis dumerilii and Platynereis cfr massiliensis at the shallow CO2 vents off Ischia, Italy

Wäge, Janine; Rotchell, Jeanette M.; Gambi, Maria-Cristina; Hardege, Jörg D.

Authors

Janine Wäge

Maria-Cristina Gambi



Contributors

Abstract

© 2017 Elsevier Ltd Many studies predict negative effects of ocean acidification on marine organisms, potentially leading to loss of biodiversity and ecosystem function. Research on species inhabiting naturally high pCO2 environments, such as volcanic CO2 vents, offers an opportunity to understand the molecular mechanisms involved in high pCO2 regulation. Here we investigate the relative expression of NADH dehydrogenase, sodium-hydrogen antiporter (NHE), carbonic anhydrase (CA) and paramyosin genes from two non-calcifying sibling Nereididae polychaetes species, Platynereis cfr massiliensis, collected in the shallow CO2 vents off Ischia (Italy; 40°43′52.0″N 13°57′46.2″E and 40°43′55.5″N 13°57′48.4″E), and P. dumerilii collected in an area nearby (40°43′34.51″N; 13°57′35.7″E). The origin of the worms was confirmed using restriction enzyme digest. NHE and paramyosin expressions were both significantly increased in P. dumerilii relative to the P. cfr massiliensis vent populations. Furthermore, a seven day laboratory transfer experiment to lower/higher pCO2 conditions was conducted to investigate the effects on the short term gene expression. The transfer experiment of the non-vent worms to high pCO2 conditions showed no significant effect on any of the genes analysed, however, two genes (NADH dehydrogenase and NHE) from worms of the vent population were significantly down-regulated under low pCO2. These findings will help to gain further insights into the cellular mechanisms affected by pCO2 changes in two polychaete species.

Journal Article Type Article
Publication Date Jul 31, 2018
Journal Estuarine, Coastal and Shelf Science
Print ISSN 0272-7714
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 207
Pages 351-358
Institution Citation Wäge, J., Rotchell, J. M., Gambi, M., & Hardege, J. D. (2018). Target gene expression studies on Platynereis dumerilii and Platynereis cfr massiliensis at the shallow CO2 vents off Ischia, Italy. Estuarine, coastal and shelf science, 207, 351-358. doi:10.1016/j.ecss.2017.11.012
DOI https://doi.org/10.1016/j.ecss.2017.11.012
Keywords Aquatic Science; Oceanography
Publisher URL https://www.sciencedirect.com/science/article/pii/S0272771416305418