Karthic Swaminathan
A Cdc42- and Rac-interactive binding (CRIB) domain mediates functions of coronin
Swaminathan, Karthic; Müller-Taubenberger, Annette; Faix, Jan; Rivero, Francisco; Noegel, Angelika A.
Authors
Annette Müller-Taubenberger
Jan Faix
Dr Francisco Rivero Crespo F.Rivero-Crespo@hull.ac.uk
Reader in Biomedical Science
Angelika A. Noegel
Abstract
The Cdc42- and Rac-interactive binding motif (CRIB) of coronin binds to Rho GTPases with a preference for GDP-loaded Rac. Mutation of the Cdc42- and Rac-interactive binding motif abrogates Rac binding. This results in increased 1evels of activated Rac in coronin-deficient Dictyostelium cells (corA - ), which impacts myosin II assembly. corA - cells show increased accumulation of myosin II in the cortex of growth-phase cells. Myosin II assembly is regulated by myosin heavy chain kinase-mediated phosphorylation of its tail. Kinase activity depends on the activation state of the p21-activated kinase a. The myosin II defect of corA - mutant is alleviated by dominantnegative p21-activated kinase a. It is rescued by wild-type coronin, whereas coronin carrying a mutated Cdc42- and Rac-interactive binding motif failed to rescue the myosin defect in corA - mutant cells. Ectopically expressed myosin heavy chain kinases affinity purified from corA - cells show reduced kinase activity. We propose that coronin through its affinity for GDP-Rac regulates the availability of GTP-Rac for activation of downstream effectors.
Citation
Swaminathan, K., Müller-Taubenberger, A., Faix, J., Rivero, F., & Noegel, A. A. (2014). A Cdc42- and Rac-interactive binding (CRIB) domain mediates functions of coronin. Proceedings of the National Academy of Sciences of the United States of America, 111(1), E25-E33. https://doi.org/10.1073/pnas.1315368111
| Journal Article Type | Article |
|---|---|
| Acceptance Date | Nov 20, 2013 |
| Online Publication Date | Dec 17, 2013 |
| Publication Date | Jan 16, 2014 |
| Deposit Date | Feb 26, 2021 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Print ISSN | 0027-8424 |
| Publisher | National Academy of Sciences |
| Peer Reviewed | Peer Reviewed |
| Volume | 111 |
| Issue | 1 |
| Pages | E25-E33 |
| DOI | https://doi.org/10.1073/pnas.1315368111 |
| Keywords | Rac GTPases; Protein–protein interaction; Cytoskeleton; Cell signaling |
| Public URL | https://hull-repository.worktribe.com/output/540870 |
| Related Public URLs | https://bradscholars.brad.ac.uk/handle/10454/17710 |
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