An attenuating role of a WASP-related protein, WASP-B, in the regulation of F-actin polymerization and pseudopod formation via the regulation of RacC during Dictyostelium chemotaxis

Chung, Chang Y.; Feoktistov, Alexander; Hollingsworth, Ryan J.; Rivero, Francisco; Mandel, Nicole S.

Abstract

The WASP family of proteins has emerged as important regulators that connect multiple signaling pathways to regulate the actin cytoskeleton. Dictyostelium cells express WASP, as well as a WASP related protein, WASP-B, endoded by wasB gene. WASP-B contains many of the domains present in WASP. Analysis of wild type, wasB null cells revealed that WASP-B is required for proper control of F-actin polymerization in response to a cAMP gradient. Due to the lack of tight control on actin polymerization, wasB null cells exhibited higher level of F-actin polymerization. wasB - cells extend more de novo pseudopods laterally and their average life span is longer than those of wild type cells, causing more turns and inefficient chemotaxis. YFP-WASP-B appears to be uniformly distributed in the cytosol and shows no translocation to cortical membrane upon cAMP stimulation. Active RacC pull-down assay reveals that the level of active RacC in wasB - cells is significantly higher than wild type cells. Moreover, the distribution of active RacC is not localized in wasB - cells. We conclude that chemotaxis defects of wasB - cells are likely to result from the aberrant regulation of RacC activation and localization. © 2013 Elsevier Inc.

Citation

Chung, C. Y., Feoktistov, A., Hollingsworth, R. J., Rivero, F., & Mandel, N. S. (2013). An attenuating role of a WASP-related protein, WASP-B, in the regulation of F-actin polymerization and pseudopod formation via the regulation of RacC during Dictyostelium chemotaxis. Biochemical and biophysical research communications, 436(4), 719-724. https://doi.org/10.1016/j.bbrc.2013.06.022

• Journal Article Type: Article
• Online Publication Date: Jun 17, 2013
• Publication Date: Jul 12, 2013
• Deposit Date: Apr 1, 2022
• Journal: Biochemical and Biophysical Research Communications
• Print ISSN: 0006-291X
• Electronic ISSN: 1090-2104
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 436
• Issue: 4
• Pages: 719-724
• DOI: https://doi.org/10.1016/j.bbrc.2013.06.022
• Public URL: https://hull-repository.worktribe.com/output/540883