Katarzyna Plak
GxcC connects Rap and Rac signaling during Dictyostelium development
Plak, Katarzyna; Veltman, Douwe; Fusetti, Fabrizia; Beeksma, Jetze; Rivero, Francisco; Van Haastert, Peter J.M.; Kortholt, Arjan
Authors
Douwe Veltman
Fabrizia Fusetti
Jetze Beeksma
Dr Francisco Rivero Crespo F.Rivero-Crespo@hull.ac.uk
Reader in Biomedical Science
Peter J.M. Van Haastert
Arjan Kortholt
Abstract
Background: Rap proteins belong to the Ras family of small G-proteins. Dictyostelium RapA is essential and implicated in processes throughout the life cycle. In early development and chemotaxis competent cells RapA induces pseudopod formation by activating PI3K and it regulates substrate attachment and myosin disassembly via the serine/threonine kinase Phg2. RapA is also important in late development, however so far little is known about the downstream effectors of RapA that play a role in this process.Results: Here we show that cells expressing constitutively active RapA exhibit a high level of Rac activation. With a pull-down screen coupled to mass spectrometry, we identified the Rac specific guanine nucleotide exchange factor, GxcC, as Rap binding partner. GxcC binds directly and specifically to active RapA and binds to a subset of Dictyostelium Rac proteins. Deletion studies revealed that this pathway is involved in regulating Dictyostelium development.Conclusions: GxcC provides a novel link between Rap and Rac signalling and is one of the Rap effectors regulating the progression of multicellular development. © 2013 Plak et al.; licensee BioMed Central Ltd.
Citation
Plak, K., Veltman, D., Fusetti, F., Beeksma, J., Rivero, F., Van Haastert, P. J., & Kortholt, A. (2013). GxcC connects Rap and Rac signaling during Dictyostelium development. BMC cell biology, 14(1), Article 6. https://doi.org/10.1186/1471-2121-14-6
Journal Article Type | Article |
---|---|
Acceptance Date | Jan 25, 2013 |
Online Publication Date | Jan 30, 2013 |
Publication Date | Jan 30, 2013 |
Deposit Date | Apr 1, 2022 |
Publicly Available Date | Apr 12, 2022 |
Journal | BMC Cell Biology |
Electronic ISSN | 1471-2121 |
Publisher | Springer Verlag |
Peer Reviewed | Peer Reviewed |
Volume | 14 |
Issue | 1 |
Article Number | 6 |
DOI | https://doi.org/10.1186/1471-2121-14-6 |
Keywords | Rap; Rac; GEF; Development; Dictyostelium |
Public URL | https://hull-repository.worktribe.com/output/540898 |
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Copyright Statement
© 2013 Plak et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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