Regulation of the actin cytoskeleton by an interaction of IQGAP related protein GAPA with Filamin and Cortexillin I

Mondal, Subhanjan; Burgute, Bhagyashri; Rieger, Daniela; Müller, Rolf; Rivero, Francisco; Faix, Jan; Schleicher, Michael; Noegel, Angelika A.

Abstract

Filamin and Cortexillin are F-actin crosslinking proteins in Dictyostelium discoideum allowing actin filaments to form three-dimensional networks. GAPA, an IQGAP related protein, is required for cytokinesis and localizes to the cleavage furrow during cytokinesis. Here we describe a novel interaction with Filamin which is required for cytokinesis and regulation of the F-actin content. The interaction occurs through the actin b inding domain of Filamin and the GRD domain of GAPA. A similar interaction takes place with Cortexillin I. We further report that Filamin associates with Rac1a implying that filamin might act as a scaffold for small GTPases. Filamin and activated Rac associate with GAPA to regulate actin remodelling. Overexpression of filamin and GAPA in the various strains suggests that GAPA regulates the actin cytoskeleton through interaction with Filamin and that it controls cytokinesis through association with Filamin and Cortexillin. © 2010 Marko et al.

Citation

Mondal, S., Burgute, B., Rieger, D., Müller, R., Rivero, F., Faix, J., Schleicher, M., & Noegel, A. A. (2010). Regulation of the actin cytoskeleton by an interaction of IQGAP related protein GAPA with Filamin and Cortexillin I. PLoS ONE, 5(11), Article e15440. https://doi.org/10.1371/journal.pone.0015440

• Journal Article Type: Article
• Acceptance Date: Sep 21, 2010
• Online Publication Date: Nov 10, 2010
• Publication Date: Dec 9, 2010
• Deposit Date: Apr 1, 2022
• Publicly Available Date: Apr 21, 2022
• Journal: PLoS ONE
• Print ISSN: 1932-6203
• Publisher: Public Library of Science
• Peer Reviewed: Peer Reviewed
• Volume: 5
• Issue: 11
• Article Number: e15440
• DOI: https://doi.org/10.1371/journal.pone.0015440
• Public URL: https://hull-repository.worktribe.com/output/541017