Skip to main content

Functional diversity of cytomegalovirus-specific T cells is maintained in older people and significantly associated with protein specificity and response size

Bajwa, Martha; Vita, Serena; Vescovini, Rosanna; Larsen, Martin; Sansoni, Paolo; Terrazzini, Nadia; Caserta, Stefano; Thomas, David; Davies, Kevin A.; Smith, Helen; Kern, Florian

Authors

Martha Bajwa

Serena Vita

Rosanna Vescovini

Martin Larsen

Paolo Sansoni

Nadia Terrazzini

David Thomas

Kevin A. Davies

Helen Smith

Florian Kern



Abstract

© The Author 2016. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. Background. Parallel upregulation of several T-cell effector functions (ie, polyfunctionality) is believed to be critical for the protection against viruses but thought to decrease in large T-cell expansions, in particular at older ages. The factors determining T-cell polyfunctionality are incompletely understood. Here we revisit the question of cytomegalovirus (CMV)-specific T-cell polyfunctionality, including a wide range of T-cell target proteins, response sizes, and participant ages. Methods. Polychromatic flow cytometry was used to analyze the functional diversity (ie, CD107, CD154, interleukin 2, tumor necrosis factor, and interferon γ expression) of CD4 + and CD8 + T-cell responses to 19 CMV proteins in a large group of young and older United Kingdom participants. A group of oldest old people (age > 85 years) was included to explore these parameters in exceptional survivors. Polyfunctionality was assessed for each protein-specific response subset, by subset and in aggregate, across all proteins by using the novel polyfunctionality index. Results. Polyfunctionality was not reduced in healthy older people as compared to young people. However, it was significantly related to target protein specificity. For each protein, it increased with response size. In the oldest old group, overall T-cell polyfunctionality was significantly lower. Discussion. Our results give a new perspective on T-cell polyfunctionality and raise the question of whether maintaining polyfunctionality of CMV-specific T cells at older ages is necessarily beneficial.

Journal Article Type Article
Publication Date Nov 1, 2016
Journal Journal of Infectious Diseases
Print ISSN 0022-1899
Electronic ISSN 1537-6613
Publisher Oxford University Press (OUP)
Peer Reviewed Peer Reviewed
Volume 214
Issue 9
Pages 1430-1437
APA6 Citation Bajwa, M., Vita, S., Vescovini, R., Larsen, M., Sansoni, P., Terrazzini, N., …Kern, F. (2016). Functional diversity of cytomegalovirus-specific T cells is maintained in older people and significantly associated with protein specificity and response size. The journal of infectious diseases : official publication of the Infectious Diseases Society of America, 214(9), 1430-1437. https://doi.org/10.1093/infdis/jiw371
DOI https://doi.org/10.1093/infdis/jiw371
Keywords Immunology and Allergy; Infectious Diseases
Publisher URL https://academic.oup.com/jid/article/214/9/1430/2576490
Related Public URLs http://sro.sussex.ac.uk/67453/
Copyright Statement © The Author 2016. Published by Oxford University Press for the Infectious Diseases Society of America.
This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (http://creativecommons....icenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, contact journals.permissions@oup.com.

Files

Article (457 Kb)
PDF

Copyright Statement
© The Author 2016. Published by Oxford University Press for the Infectious Diseases Society of America.
This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, contact journals.permissions@oup.com.



You might also like



Downloadable Citations

;