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Induction of human regulatory T cells with bacterial superantigens

Caserta, Stefano; Taylor, Amanda L.; Terrazzini, Nadia; Llewelyn, Martin J.

Authors

Amanda L. Taylor

Nadia Terrazzini

Martin J. Llewelyn



Abstract

© Springer Science+Business Media New York 2016. Regulatory T cells (Tregs) that suppress the activation of immune effector cells limit immunopathology and are fast emerging as therapeutic targets for autoimmune and cancer disease. Tools enabling Treg in vitro -induction, expansion, and characterization and manipulation will help future clinical developments. In this chapter, we describe in detail how to use bacterial superantigens to induce human Tregs effi ciently from peripheral blood mononuclear cells. How to assess human Treg phenotype and suppressive capacity are also described. Technical details, variations, and alternative experimental conditions are provided.

Publication Date Jan 1, 2016
Volume 1396
Pages 181-206
Series Title Superantigens. Methods in Molecular Biology
Book Title Superantigens; Methods in Molecular Biology
Chapter Number 16
ISBN 9781493933426; 9781493933440
APA6 Citation Caserta, S., Taylor, A. L., Terrazzini, N., & Llewelyn, M. J. (2016). Induction of human regulatory T cells with bacterial superantigens. In Superantigens; Methods in Molecular Biology, 181-206. https://doi.org/10.1007/978-1-4939-3344-0_16
DOI https://doi.org/10.1007/978-1-4939-3344-0_16
Keywords Superantigen; Regulatory T cells; Human; Superantigen stimulation; Treg phenotype; Suppression assay; Cell purification; PBMC; Immunological methods
Publisher URL https://link.springer.com/protocol/10.1007%2F978-1-4939-3344-0_16
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