Skip to main content

Research Repository

Advanced Search

Induction of human regulatory T cells with bacterial superantigens

Caserta, Stefano; Taylor, Amanda L.; Terrazzini, Nadia; Llewelyn, Martin J.


Amanda L. Taylor

Nadia Terrazzini

Martin J. Llewelyn


© Springer Science+Business Media New York 2016. Regulatory T cells (Tregs) that suppress the activation of immune effector cells limit immunopathology and are fast emerging as therapeutic targets for autoimmune and cancer disease. Tools enabling Treg in vitro -induction, expansion, and characterization and manipulation will help future clinical developments. In this chapter, we describe in detail how to use bacterial superantigens to induce human Tregs effi ciently from peripheral blood mononuclear cells. How to assess human Treg phenotype and suppressive capacity are also described. Technical details, variations, and alternative experimental conditions are provided.


Caserta, S., Taylor, A. L., Terrazzini, N., & Llewelyn, M. J. (2016). Induction of human regulatory T cells with bacterial superantigens. In Superantigens; Methods in Molecular Biology (181-206).

Acceptance Date Dec 17, 2015
Online Publication Date Dec 17, 2015
Publication Date Jan 1, 2016
Deposit Date Jun 28, 2018
Volume 1396
Pages 181-206
Series Title Superantigens. Methods in Molecular Biology
Book Title Superantigens; Methods in Molecular Biology
Chapter Number 16
ISBN 9781493933426; 9781493933440
Keywords Superantigen; Regulatory T cells; Human; Superantigen stimulation; Treg phenotype; Suppression assay; Cell purification; PBMC; Immunological methods
Public URL
Publisher URL