Induction of human regulatory T cells with bacterial superantigens

Caserta, Stefano; Taylor, Amanda L.; Terrazzini, Nadia; Llewelyn, Martin J.

Abstract

© Springer Science+Business Media New York 2016. Regulatory T cells (Tregs) that suppress the activation of immune effector cells limit immunopathology and are fast emerging as therapeutic targets for autoimmune and cancer disease. Tools enabling Treg in vitro -induction, expansion, and characterization and manipulation will help future clinical developments. In this chapter, we describe in detail how to use bacterial superantigens to induce human Tregs effi ciently from peripheral blood mononuclear cells. How to assess human Treg phenotype and suppressive capacity are also described. Technical details, variations, and alternative experimental conditions are provided.

Citation

Caserta, S., Taylor, A. L., Terrazzini, N., & Llewelyn, M. J. (2016). Induction of human regulatory T cells with bacterial superantigens. In Superantigens; Methods in Molecular Biology (181-206). https://doi.org/10.1007/978-1-4939-3344-0_16

• Acceptance Date: Dec 17, 2015
• Online Publication Date: Dec 17, 2015
• Publication Date: Jan 1, 2016
• Deposit Date: Jun 28, 2018
• Volume: 1396
• Pages: 181-206
• Series Title: Superantigens. Methods in Molecular Biology
• Book Title: Superantigens; Methods in Molecular Biology
• Chapter Number: 16
• ISBN: 9781493933426; 9781493933440
• DOI: https://doi.org/10.1007/978-1-4939-3344-0_16
• Keywords: Superantigen; Regulatory T cells; Human; Superantigen stimulation; Treg phenotype; Suppression assay; Cell purification; PBMC; Immunological methods
• Public URL: https://hull-repository.worktribe.com/output/620535
• Publisher URL: https://link.springer.com/protocol/10.1007%2F978-1-4939-3344-0_16