Dr Stefano Caserta S.Caserta@hull.ac.uk
Lecturer in Immunology
IL-7 is superior to IL-2 for ex vivo expansion of tumourspecific CD4 + T cells
Caserta, Stefano; Alessi, Patrizia; Basso, Veronica; Mondino, Anna
Authors
Patrizia Alessi
Veronica Basso
Anna Mondino
Abstract
It is well established that tumours hinder both natural and vaccine-induced tumourspecific CD4 + T-cell responses. Adoptive T-cell therapy has the potential to circumvent functional tolerance and enhance anti-tumour protective responses. While protocols suitable for the expansion of cytotoxic CD8 + T cells are currently available, data on tumour-specific CD4 + T cells remain scarce.We report here that CD4 + T cells sensitized to tumour-associated Ag in vivo, proliferate in vitro in response to IL-7 without the need for exogenous Ag stimulation and accumulate several folds while preserving a memory-like phenotype. Both cell proliferation and survival accounts for the outgrowth of tumoursensitized T cells among other memory and naive lymphocytes following exposure to IL-7. Also IL-2, previously used to expand anti-tumour CTL, promotes tumour-specific CD4 + T-cell accumulation. However, IL-7 is superior to IL-2 at preserving lymphocyte viability, in vitro and in vivo, maintaining those properties, that are required by helper CD4 + T cells to confer therapeutic efficacy upon transplantation in tumour-bearing hosts. Together our data support a unique role for IL-7 in retrieving memory-like CD4 + T cells suitable for adoptive T-cell therapy. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.
Citation
Caserta, S., Alessi, P., Basso, V., & Mondino, A. (2010). IL-7 is superior to IL-2 for ex vivo expansion of tumourspecific CD4 + T cells. European journal of immunology, 40(2), 470-479. https://doi.org/10.1002/eji.200939801
| Journal Article Type | Article |
|---|---|
| Acceptance Date | Nov 12, 2009 |
| Online Publication Date | Nov 30, 2009 |
| Publication Date | Feb 1, 2010 |
| Deposit Date | Jun 28, 2018 |
| Journal | European Journal of Immunology |
| Print ISSN | 0014-2980 |
| Publisher | Wiley |
| Peer Reviewed | Peer Reviewed |
| Volume | 40 |
| Issue | 2 |
| Pages | 470-479 |
| DOI | https://doi.org/10.1002/eji.200939801 |
| Keywords | Adoptive T‐cell therapy; Cytokines; T helper cells; Tumour immunology |
| Public URL | https://hull-repository.worktribe.com/output/620565 |
| Publisher URL | https://onlinelibrary.wiley.com/doi/abs/10.1002/eji.200939801 |