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A novel silver bioactive glass elicits antimicrobial efficacy against Pseudomonas aeruginosa and Staphylococcus aureus in an ex vivo skin wound biofilm model

Wilkinson, Holly N.; Iveson, Sammi; Catherall, Paul; Hardman, Matthew J.

Authors

Sammi Iveson

Paul Catherall



Abstract

Biofilm infection is now understood to be a potent contributor to the recalcitrant nature of chronic wounds. Bacterial biofilms evade the host immune response and show increased resistance to antibiotics. Along with improvements in antibiotic stewardship, effective new anti-biofilm therapies are urgently needed for effective wound management. Previous studies have shown that bioactive glass (Bg) is able to promote healing with moderate bactericidal activity. Here we tested the antimicrobial efficacy of a novel BG incorporating silver (BgAg), against both planktonic and biofilm forms of the wound-relevant bacteria Pseudomonas aeruginosa and Staphylococcus aureus. BgAg was stable, long lasting, and potently effective against planktonic bacteria in time-kill assays (6-log reduction in bacterial viability within 2 h) and in agar diffusion assays. BgAg reduced bacterial load in a physiologically relevant ex vivo porcine wound biofilm model; P. aeruginosa (2-log reduction) and S. aureus (3-log reduction). BgAg also conferred strong effects against P. aeruginosa biofilm virulence, reducing both protease activity and virulence gene expression. Co-culture biofilms appeared more resistant to BgAg, where a selective reduction in S. aureus was observed. Finally, BgAg was shown to benefit the host response to biofilm infection, directly reducing host tissue cell death. Taken together, the findings provide evidence that BgAg elicits potent antimicrobial effects against planktonic and single-species biofilms, with beneficial effects on the host tissue response. Further investigations are required to elucidate the specific consequences of BG administration on polymicrobial biofilms, and further explore the effects on host–microbe interactions.

Citation

Wilkinson, H. N., Iveson, S., Catherall, P., & Hardman, M. J. (2018). A novel silver bioactive glass elicits antimicrobial efficacy against Pseudomonas aeruginosa and Staphylococcus aureus in an ex vivo skin wound biofilm model. Frontiers in Microbiology, 9(JUL), Article 1450. https://doi.org/10.3389/fmicb.2018.01450

Journal Article Type Article
Acceptance Date Jun 11, 2018
Online Publication Date Jul 3, 2018
Publication Date Jul 3, 2018
Deposit Date Jul 9, 2018
Publicly Available Date Jul 9, 2018
Journal Frontiers in Microbiology
Print ISSN 1664-302X
Electronic ISSN 1664-302X
Publisher Frontiers Media
Peer Reviewed Peer Reviewed
Volume 9
Issue JUL
Article Number 1450
DOI https://doi.org/10.3389/fmicb.2018.01450
Keywords Microbiology (medical); Microbiology
Public URL https://hull-repository.worktribe.com/output/916814
Publisher URL https://www.frontiersin.org/articles/10.3389/fmicb.2018.01450/full

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Copyright Statement
© 2018 Wilkinson, Iveson, Catherall and Hardman. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.







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