All Outputs (918)

Multiplexed phosphospecific flow cytometry enables large-scale signaling profiling and drug screening in blood platelets (2014)
Journal Article
Spurgeon, B. E., Aburima, A., Oberprieler, N. G., Taskén, K., & Naseem, K. M. (2014). Multiplexed phosphospecific flow cytometry enables large-scale signaling profiling and drug screening in blood platelets. Journal of thrombosis and haemostasis : JTH, 12(10), 1733-1743. https://doi.org/10.1111/jth.12670

Background: Dissecting the signaling events that contribute to platelet activation will increase our understanding of platelet function and aid in the development of new antiplatelet agents. However, high-throughput methodology for the quantitative a... Read More about Multiplexed phosphospecific flow cytometry enables large-scale signaling profiling and drug screening in blood platelets.

RASSF1A–LATS1 signalling stabilizes replication forks by restricting CDK2-mediated phosphorylation of BRCA2 (2014)
Journal Article
Pefani, D.-E., Latusek, R., Pires, I., Grawenda, A. M., Yee, K. S., Hamilton, G., van der Weyden, L., Esashi, F., Hammond, E. M., & O'Neill, E. (2014). RASSF1A–LATS1 signalling stabilizes replication forks by restricting CDK2-mediated phosphorylation of BRCA2. Nature Cell Biology, 16(10), 962-971. https://doi.org/10.1038/ncb3035

Genomic instability is a key hallmark of cancer leading to tumour heterogeneity and therapeutic resistance. ​BRCA2 has a fundamental role in error-free DNA repair but also sustains genome integrity by promoting ​RAD51 nucleofilament formation at stal... Read More about RASSF1A–LATS1 signalling stabilizes replication forks by restricting CDK2-mediated phosphorylation of BRCA2.

Apolipoprotein E ε4 allele modulates the immediate impact of acute exercise on prefrontal function (2014)
Journal Article
De Marco, M., Clough, P. J., Dyer, C. E., Vince, R. V., Waby, J. S., Midgley, A. W., & Venneri, A. (2015). Apolipoprotein E ε4 allele modulates the immediate impact of acute exercise on prefrontal function. Behavior Genetics, 45(1), 106-116. https://doi.org/10.1007/s10519-014-9675-5

The difference between Apolipoprotein E ε4 carriers and non-carriers in response to single exercise sessions was tested. Stroop and Posner tasks were administered to young untrained women immediately after walking sessions or moderately heavy exercis... Read More about Apolipoprotein E ε4 allele modulates the immediate impact of acute exercise on prefrontal function.

Identification of N-terminal protein acetylation and arginine methylation of the voltage-gated sodium channel in end-stage heart failure human heart (2014)
Journal Article
Beltran-Alvarez, P., Tarradas, A., Chiva, C., Pérez-Serra, A., Batlle, M., Pérez-Villa, F., Schulte, U., Sabidó, E., Brugada, R., & Pagans, S. (2014). Identification of N-terminal protein acetylation and arginine methylation of the voltage-gated sodium channel in end-stage heart failure human heart. Journal of Molecular and Cellular Cardiology, 76, 126-129. https://doi.org/10.1016/j.yjmcc.2014.08.014

The α subunit of the cardiac voltage-gated sodium channel, Naᵥ1.5, provides the rapid sodium inward current that initiates cardiomyocyte action potentials. Here, we analyzed for the first time the post-translational modifications of Naᵥ1.5 purified f... Read More about Identification of N-terminal protein acetylation and arginine methylation of the voltage-gated sodium channel in end-stage heart failure human heart.

Absence of plastin 1 causes abnormal maintenance of hair cell stereocilia and a moderate form of hearing loss in mice (2014)
Journal Article
Taylor, R., Bullen, A., Johnson, S. L., Grimm-Günter, E. M., Rivero, F., Marcotti, W., Forge, A., & Daudet, N. (2015). Absence of plastin 1 causes abnormal maintenance of hair cell stereocilia and a moderate form of hearing loss in mice. Human molecular genetics, 24(1), 37-49. https://doi.org/10.1093/hmg/ddu417

© The Author 2014. Published by Oxford University Press. All rights reserved. Hearing relies on the mechanosensory inner and outer hair cells (OHCs) of the organ of Corti, which convert mechanical deflections of their actin-rich stereociliary bundles... Read More about Absence of plastin 1 causes abnormal maintenance of hair cell stereocilia and a moderate form of hearing loss in mice.

Frequency over function : raised levels of CD127low/- regulatory T cells in the tumour microenvironment compared with the periphery of head and neck cancer patients (2014)
Journal Article
Drennan, S., Stafford, N. D., Greenman, J., & Green, V. L. (2014). Frequency over function : raised levels of CD127low/- regulatory T cells in the tumour microenvironment compared with the periphery of head and neck cancer patients. Journal of clinical & cellular immunology, 5(4), Article 241. https://doi.org/10.4172/2155-9899.1000241

Objective: Regulatory T cells (Tregs) are known to infiltrate the tumour microenvironment of many cancers, including head and neck malignancies, and are thought to contribute to the host's impaired anti-tumour immune response. However, their immunosu... Read More about Frequency over function : raised levels of CD127low/- regulatory T cells in the tumour microenvironment compared with the periphery of head and neck cancer patients.

PGC1β activates an antiangiogenic program to repress neoangiogenesis in muscle ischemia (2014)
Journal Article
Yadav, V., Matsakas, A., Lorca, S., & Narkar, V. A. (2014). PGC1β activates an antiangiogenic program to repress neoangiogenesis in muscle ischemia. Cell reports, 8(3), 783-797. https://doi.org/10.1016/j.celrep.2014.06.040

Revascularization of ischemic skeletalmuscle is governed by a balance between pro- and antiangiogenic factors in multiple cell types but particularly in myocytes and endothelial cells. Whereas the regulators of proangiogenic factors are well defined... Read More about PGC1β activates an antiangiogenic program to repress neoangiogenesis in muscle ischemia.

HIF-1 alpha-independent hypoxia-induced rapid PTK6 stabilization is associated with increased motility and invasion (2014)
Journal Article
Poujade, F.-A., Harvey, A. J., Blokland, N. J. G., Broos, A. W. T., Eccles, S. A., Hammond, E. M., Pires, I. M., Senra, J. M., & Span, P. N. (2014). HIF-1 alpha-independent hypoxia-induced rapid PTK6 stabilization is associated with increased motility and invasion. Cancer Biology and Therapy, 15(10), 1350-1357. https://doi.org/10.4161/cbt.29822

© 2014 Landes Bioscience. PTK6/Brk is a non-receptor tyrosine kinase overexpressed in cancer. Here we demonstrate that cytosolic PTK6 is rapidly and robustly induced in response to hypoxic conditions in a HIF-1-independent manner. Furthermore, a prop... Read More about HIF-1 alpha-independent hypoxia-induced rapid PTK6 stabilization is associated with increased motility and invasion.

Clinical response to primary letrozole therapy in elderly patients with early breast cancer : possible role for p53 as a biomarker (2014)
Journal Article
Garimella, V., Hussain, T., Agarwal, V., Radhakrishna, S., Fox, J. N., Kneeshaw, P. J., Long, E. D., Mahapatra, T. K., McManus, P. L., Lind, M. J., Drew, P. J., & Cawkwell, L. (2014). Clinical response to primary letrozole therapy in elderly patients with early breast cancer : possible role for p53 as a biomarker. International journal of surgery, 12(8), 821-826. https://doi.org/10.1016/j.ijsu.2014.06.009

Primary tamoxifen therapy has been widely used to treat elderly women with ER-positive breast cancer in the past. Aromatase inhibitors may be more beneficial than tamoxifen when used as primary endocrine therapy in elderly patients. We aimed to retro... Read More about Clinical response to primary letrozole therapy in elderly patients with early breast cancer : possible role for p53 as a biomarker.

Telomere dysfunction accurately predicts clinical outcome in chronic lymphocytic leukaemia, even in patients with early stage disease (2014)
Journal Article
Lin, T. T., Norris, K., Heppel, N. H., Pratt, G., Allan, J. M., Allsup, D. J., Bailey, J., Cawkwell, L., Hills, R., Grimstead, J. W., Jones, R. E., Britt-Compton, B., Fegan, C., Baird, D. M., & Pepper, C. (2014). Telomere dysfunction accurately predicts clinical outcome in chronic lymphocytic leukaemia, even in patients with early stage disease. British journal of haematology, 167(2), 214-223. https://doi.org/10.1111/bjh.13023

© 2014 John Wiley & Sons Ltd. Defining the prognosis of individual cancer sufferers remains a significant clinical challenge. Here we assessed the ability of high-resolution single telomere length analysis (STELA), combined with an experimentally d... Read More about Telomere dysfunction accurately predicts clinical outcome in chronic lymphocytic leukaemia, even in patients with early stage disease.

Human TRPM8 and TRPA1 pain channels, including a gene variant with increased sensitivity to agonists (TRPA1 R797T), exhibit differential regulation by SRC-tyrosine kinase inhibitor (2014)
Journal Article
Morgan, K., Sadofsky, L. R., Crow, C., & Morice, A. H. (2014). Human TRPM8 and TRPA1 pain channels, including a gene variant with increased sensitivity to agonists (TRPA1 R797T), exhibit differential regulation by SRC-tyrosine kinase inhibitor. Bioscience Reports, 34(4), 469-478. https://doi.org/10.1042/BSR20140061

TRPM8 (transient receptor potential M8) and TRPA1 (transient receptor potential A1) are cold-temperature-sensitive nociceptors expressed in sensory neurons but their behaviour in neuronal cells is poorly understood. Therefore DNA expression construct... Read More about Human TRPM8 and TRPA1 pain channels, including a gene variant with increased sensitivity to agonists (TRPA1 R797T), exhibit differential regulation by SRC-tyrosine kinase inhibitor.

Amino acids in the uterine luminal fluid reflects the temporal changes in transporter expression in the endometrium and conceptus during early pregnancy in cattle (2014)
Journal Article
Forde, N., Simintiras, C. A., Sturmey, R., Mamo, S., Kelly, A. K., Spencer, T. E., Bazer, F. W., & Lonergan, P. (2014). Amino acids in the uterine luminal fluid reflects the temporal changes in transporter expression in the endometrium and conceptus during early pregnancy in cattle. PLoS ONE, 9(6), e100010. https://doi.org/10.1371/journal.pone.0100010

In cattle, conceptus-maternal interactions are critical for the establishment and maintenance of pregnancy. A major component of this early interaction involves the transport of nutrients and secretion of key molecules by uterine epithelial cells to... Read More about Amino acids in the uterine luminal fluid reflects the temporal changes in transporter expression in the endometrium and conceptus during early pregnancy in cattle.

Expression analysis of mouse Rhobtb3 using a LacZ reporter and preliminary characterization of a knockout strain (2014)
Journal Article
Lutz, J., Grimm-Günter, E. M. S., Joshi, P., & Rivero, F. (2014). Expression analysis of mouse Rhobtb3 using a LacZ reporter and preliminary characterization of a knockout strain. Histochemistry and cell biology, 142(5), 511-528. https://doi.org/10.1007/s00418-014-1235-9

RhoBTB3 is an atypical member of the Rho family of small GTPases. It localizes at the Golgi apparatus and endosomes and is involved in vesicle trafficking and in targeting proteins for degradation in the proteasome. Previous studies using Northern bl... Read More about Expression analysis of mouse Rhobtb3 using a LacZ reporter and preliminary characterization of a knockout strain.

Oral tolerance to cancer can be abrogated by T regulatory cell inhibition (2014)
Journal Article
Whelan, M. C., Casey, G., Larkin, J. O., Guinn, B. A., O'Sullivan, G. C., & Tangney, M. (2014). Oral tolerance to cancer can be abrogated by T regulatory cell inhibition. PLoS ONE, 9(5), Article e97602. https://doi.org/10.1371/journal.pone.0097602

Oral administration of tumour cells induces an immune hypo-responsiveness known as oral tolerance. We have previously shown that oral tolerance to a cancer is tumour antigen specific, non-cross-reactive and confers a tumour growth advantage. We inves... Read More about Oral tolerance to cancer can be abrogated by T regulatory cell inhibition.

Advances in processes for PET radiotracer synthesis: Separation of [18F]fluoride from enriched [18O]water (2014)
Journal Article
He, P., Haswell, S. J., Pamme, N., & Archibald, S. J. (2014). Advances in processes for PET radiotracer synthesis: Separation of [18F]fluoride from enriched [18O]water. Applied radiation and isotopes : including data, instrumentation, and methods for use in agriculture, industry, and medicine, 91, 64-70. https://doi.org/10.1016/j.apradiso.2014.04.021

Positron emission tomography (PET) is a powerful scientific and clinical tool for the study and visualization of human physiology that can provide important information about metabolism and diseases such as cancer. At present, [ 18 F]fluorodeoxygluco... Read More about Advances in processes for PET radiotracer synthesis: Separation of [18F]fluoride from enriched [18O]water.

Clinical relevance of immune parameters in the tumor microenvironment of head and neck cancers (2014)
Journal Article
Wallis, S. P., Stafford, N. D., & Greenman, J. (2015). Clinical relevance of immune parameters in the tumor microenvironment of head and neck cancers. Head & neck, 37(3), 449-459. https://doi.org/10.1002/hed.23736

© 2014 Wiley Periodicals, Inc. Background. The tumour microenvironment is a highly complex region where multiple interactions occur between host and cancer cells. The host response is defined by the presence and function of different tumour infiltrat... Read More about Clinical relevance of immune parameters in the tumor microenvironment of head and neck cancers.

Metabolic heterogeneity during preimplantation development: The missing link? (2014)
Journal Article
Brison, D. R., Sturmey, R. G., & Leese, H. J. (2014). Metabolic heterogeneity during preimplantation development: The missing link?. Human Reproduction Update, 20(5), 632-640. https://doi.org/10.1093/humupd/dmu018

BACKGROUND Most tissues in the body rely on the presence of gap junctions in order to couple their component cells electrically and metabolically via intercellular transport of ions, metabolites and signalling agents. As a result, cells within tissue... Read More about Metabolic heterogeneity during preimplantation development: The missing link?.

The visualization of biofilms in chronic diabetic foot wounds using routine diagnostic microscopy methods (2014)
Journal Article
Oates, A., Bowling, F. L., Boulton, A. J. M., Bowler, P. G., Metcalf, D. G., & McBain, A. J. (2014). The visualization of biofilms in chronic diabetic foot wounds using routine diagnostic microscopy methods. Journal of Diabetes Research, 2014, Article 153586. https://doi.org/10.1155/2014/153586

Diabetic foot wounds are commonly colonised by taxonomically diverse microbial communities and may additionally be infected with specific pathogens. Since biofilms are demonstrably less susceptible to antimicrobial agents than are planktonic bacteria... Read More about The visualization of biofilms in chronic diabetic foot wounds using routine diagnostic microscopy methods.

Estrogen receptor-alpha promotes alternative macrophage activation during cutaneous repair (2014)
Journal Article
Campbell, L., Emmerson, E., Williams, H., Saville, C. R., Krust, A., Chambon, P., Mace, K. A., & Hardman, M. J. (2014). Estrogen receptor-alpha promotes alternative macrophage activation during cutaneous repair. Journal of Investigative Dermatology, 134(9), 2447-2457. https://doi.org/10.1038/jid.2014.175

Efficient local monocyte/macrophage recruitment is critical for tissue repair. Recruited macrophages are polarized toward classical (proinflammatory) or alternative (prohealing) activation in response to cytokines, with tight temporal regulation cruc... Read More about Estrogen receptor-alpha promotes alternative macrophage activation during cutaneous repair.

Amplification of bacteria-induced platelet activation is triggered by FcγRIIA, integrin αiIbβ3, and platelet factor 4 (2014)
Journal Article
Arman, M., Krauel, K., Tilley, D. O., Weber, C., Cox, D., Greinacher, A., Kerrigan, S. W., & Watson, S. P. (2014). Amplification of bacteria-induced platelet activation is triggered by FcγRIIA, integrin αiIbβ3, and platelet factor 4. Blood, 123(20), 3166-3174. https://doi.org/10.1182/blood-2013-11-540526

Bacterial adhesion to platelets is mediated via a range of strain-specific bacterial surface proteins that bind to a variety of platelet receptors. It is unclear how these interactions lead to platelet activation. We demonstrate a critical role for t... Read More about Amplification of bacteria-induced platelet activation is triggered by FcγRIIA, integrin αiIbβ3, and platelet factor 4.