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Oral tolerance to cancer can be abrogated by T regulatory cell inhibition

Whelan, Maria C.; Casey, Garrett; Larkin, John O.; Guinn, Barbara-ann; O'Sullivan, Gerald C.; Tangney, Mark

Authors

Maria C. Whelan

Garrett Casey

John O. Larkin

Gerald C. O'Sullivan

Mark Tangney



Abstract

Oral administration of tumour cells induces an immune hypo-responsiveness known as oral tolerance. We have previously shown that oral tolerance to a cancer is tumour antigen specific, non-cross-reactive and confers a tumour growth advantage. We investigated the utilisation of regulatory T cell (Treg) depletion on oral tolerance to a cancer and its ability to control tumour growth. Balb/C mice were gavage fed homogenised tumour tissue – JBS fibrosarcoma (to induce oral tolerance to a cancer), or PBS as control. Growth of subcutaneous JBS tumours were measured; splenic tissue excised and flow cytometry used to quantify and compare systemic Tregs and T effector (Teff) cell populations. Prior to and/or following tumour feeding, mice were intraperitoneally administered anti-CD25, to inactivate systemic Tregs, or given isotype antibody as a control. Mice which were orally tolerised prior to subcutaneous tumour induction, displayed significantly higher systemic Treg levels (14% vs 6%) and faster tumour growth rates than controls (p

Journal Article Type Article
Publication Date May 15, 2014
Journal PLoS ONE
Print ISSN 1932-6203
Publisher Public Library of Science
Peer Reviewed Peer Reviewed
Volume 9
Issue 5
Article Number e97602
APA6 Citation Whelan, M. C., Casey, G., Larkin, J. O., Guinn, B., O'Sullivan, G. C., & Tangney, M. (2014). Oral tolerance to cancer can be abrogated by T regulatory cell inhibition. PloS one, 9(5), https://doi.org/10.1371/journal.pone.0097602
DOI https://doi.org/10.1371/journal.pone.0097602
Publisher URL https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0097602

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Copyright Statement
Copyright: 2014 Whelan et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.





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