Skip to main content

Oral tolerance to cancer can be abrogated by T regulatory cell inhibition

Whelan, Maria C.; Casey, Garrett; Larkin, John O.; Guinn, Barbara-ann; O'Sullivan, Gerald C.; Tangney, Mark


Maria C. Whelan

Garrett Casey

John O. Larkin

Gerald C. O'Sullivan

Mark Tangney


Oral administration of tumour cells induces an immune hypo-responsiveness known as oral tolerance. We have previously shown that oral tolerance to a cancer is tumour antigen specific, non-cross-reactive and confers a tumour growth advantage. We investigated the utilisation of regulatory T cell (Treg) depletion on oral tolerance to a cancer and its ability to control tumour growth. Balb/C mice were gavage fed homogenised tumour tissue – JBS fibrosarcoma (to induce oral tolerance to a cancer), or PBS as control. Growth of subcutaneous JBS tumours were measured; splenic tissue excised and flow cytometry used to quantify and compare systemic Tregs and T effector (Teff) cell populations. Prior to and/or following tumour feeding, mice were intraperitoneally administered anti-CD25, to inactivate systemic Tregs, or given isotype antibody as a control. Mice which were orally tolerised prior to subcutaneous tumour induction, displayed significantly higher systemic Treg levels (14% vs 6%) and faster tumour growth rates than controls (p

Journal Article Type Article
Publication Date May 15, 2014
Journal PLoS ONE
Print ISSN 1932-6203
Publisher Public Library of Science
Peer Reviewed Peer Reviewed
Volume 9
Issue 5
Article Number e97602
APA6 Citation Whelan, M. C., Casey, G., Larkin, J. O., Guinn, B., O'Sullivan, G. C., & Tangney, M. (2014). Oral tolerance to cancer can be abrogated by T regulatory cell inhibition. PloS one, 9(5),
Publisher URL


Article (1.4 Mb)

Copyright Statement
Copyright: 2014 Whelan et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

You might also like

Downloadable Citations