Oral tolerance to cancer can be abrogated by T regulatory cell inhibition

Whelan, Maria C.; Casey, Garrett; Larkin, John O.; Guinn, Barbara Ann; O'Sullivan, Gerald C.; Tangney, Mark

Abstract

Oral administration of tumour cells induces an immune hypo-responsiveness known as oral tolerance. We have previously shown that oral tolerance to a cancer is tumour antigen specific, non-cross-reactive and confers a tumour growth advantage. We investigated the utilisation of regulatory T cell (Treg) depletion on oral tolerance to a cancer and its ability to control tumour growth. Balb/C mice were gavage fed homogenised tumour tissue - JBS fibrosarcoma (to induce oral tolerance to a cancer), or PBS as control. Growth of subcutaneous JBS tumours were measured; splenic tissue excised and flow cytometry used to quantify and compare systemic Tregs and T effector (Teff) cell populations. Prior to and/or following tumour feeding, mice were intraperitoneally administered anti-CD25, to inactivate systemic Tregs, or given isotype antibody as a control. Mice which were orally tolerised prior to subcutaneous tumour induction, displayed significantly higher systemic Treg levels (14% vs 6%) and faster tumour growth rates than controls (p

Citation

Whelan, M. C., Casey, G., Larkin, J. O., Guinn, B. A., O'Sullivan, G. C., & Tangney, M. (2014). Oral tolerance to cancer can be abrogated by T regulatory cell inhibition. PLoS ONE, 9(5), Article e97602. https://doi.org/10.1371/journal.pone.0097602

• Journal Article Type: Article
• Acceptance Date: Apr 22, 2014
• Online Publication Date: May 15, 2014
• Publication Date: May 15, 2014
• Deposit Date: Apr 9, 2019
• Publicly Available Date: Apr 10, 2019
• Journal: PLoS ONE
• Print ISSN: 1932-6203
• Electronic ISSN: 1932-6203
• Publisher: Public Library of Science
• Peer Reviewed: Peer Reviewed
• Volume: 9
• Issue: 5
• Article Number: e97602
• DOI: https://doi.org/10.1371/journal.pone.0097602
• Public URL: https://hull-repository.worktribe.com/output/1567103
• Publisher URL: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0097602