Identification of the Antigens Recognised by Colorectal Cancer Patients Using Sera from Patients Who Exhibit a Crohn’s-like Lymphoid Reaction
Boncheva, Viktoriya; Linnebacher, Michael; Kdimati, Said; Draper, Hannah; Orchard, Laurence; Mills, Ken; O’Sullivan, Gerald; Tangney, Mark; Guinn, Barbara-ann
Dr Barbara Guinn B.Guinn@hull.ac.uk
Reader in Biomedical Sciences
A Crohn’s-like lymphoid reaction (CLR) is observed in about 15% of colorectal cancer (CRC) patients and is associated with favourable outcomes. To identify the immune targets recognised by CRC CLR patient sera, we immunoscreened a testes cDNA library with sera from three patients. Immunoscreening of the 18 antigens identified by SEREX with sera from normal donors showed that only the heavy chain of IgG3 (IGHG3) and a novel antigen we named UOB-COL-7, were solely recognised by sera from CRC CLR patients. ELISA showed an elevation in IgG3 levels in patients with CRC (p = 0.01). To extend our studies we analysed the expression of our SEREX-identified antigens using the RNA-sequencing dataset (GSE5206). We found that the transcript levels of multiple IGHG probesets were highly significant (p < 0.001) in their association with clinical features of CRC while above median levels of DAPK1 (p = 0.005) and below median levels of GTF2H5 (p = 0.004) and SH3RF2 (p = 0.02) were associated with improved overall survival. Our findings demonstrate the potential of SEREX-identified CRC CLR antigens to act as biomarkers for CRC and provide a rationale for their further characterization and validation.
Boncheva, V., Linnebacher, M., Kdimati, S., Draper, H., Orchard, L., Mills, K., …Guinn, B. (2022). Identification of the Antigens Recognised by Colorectal Cancer Patients Using Sera from Patients Who Exhibit a Crohn’s-like Lymphoid Reaction. Biomolecules, 12(8), 1058. https://doi.org/10.3390/biom12081058
|Journal Article Type||Article|
|Acceptance Date||Jul 25, 2022|
|Online Publication Date||Jul 29, 2022|
|Publication Date||Jul 29, 2022|
|Deposit Date||Aug 2, 2022|
|Publicly Available Date||Aug 3, 2022|
|Peer Reviewed||Peer Reviewed|
|Keywords||Colon cancer; SEREX; Crohn’s-like lymphoid reaction (CLR); Immunotherapy; Immunoglobulin heavy chain|
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Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
This article is an open access article distributed under the terms and
conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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