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Identification of the Antigens Recognised by Colorectal Cancer Patients Using Sera from Patients Who Exhibit a Crohn’s-like Lymphoid Reaction

Boncheva, Viktoriya; Linnebacher, Michael; Kdimati, Said; Draper, Hannah; Orchard, Laurence; Mills, Ken; O’Sullivan, Gerald; Tangney, Mark; Guinn, Barbara-ann

Authors

Viktoriya Boncheva

Michael Linnebacher

Said Kdimati

Hannah Draper

Laurence Orchard

Ken Mills

Gerald O’Sullivan

Mark Tangney



Abstract

A Crohn’s-like lymphoid reaction (CLR) is observed in about 15% of colorectal cancer (CRC) patients and is associated with favourable outcomes. To identify the immune targets recognised by CRC CLR patient sera, we immunoscreened a testes cDNA library with sera from three patients. Immunoscreening of the 18 antigens identified by SEREX with sera from normal donors showed that only the heavy chain of IgG3 (IGHG3) and a novel antigen we named UOB-COL-7, were solely recognised by sera from CRC CLR patients. ELISA showed an elevation in IgG3 levels in patients with CRC (p = 0.01). To extend our studies we analysed the expression of our SEREX-identified antigens using the RNA-sequencing dataset (GSE5206). We found that the transcript levels of multiple IGHG probesets were highly significant (p < 0.001) in their association with clinical features of CRC while above median levels of DAPK1 (p = 0.005) and below median levels of GTF2H5 (p = 0.004) and SH3RF2 (p = 0.02) were associated with improved overall survival. Our findings demonstrate the potential of SEREX-identified CRC CLR antigens to act as biomarkers for CRC and provide a rationale for their further characterization and validation.

Citation

Boncheva, V., Linnebacher, M., Kdimati, S., Draper, H., Orchard, L., Mills, K., …Guinn, B. (2022). Identification of the Antigens Recognised by Colorectal Cancer Patients Using Sera from Patients Who Exhibit a Crohn’s-like Lymphoid Reaction. Biomolecules, 12(8), 1058. https://doi.org/10.3390/biom12081058

Journal Article Type Article
Acceptance Date Jul 25, 2022
Online Publication Date Jul 29, 2022
Publication Date Jul 29, 2022
Deposit Date Aug 2, 2022
Publicly Available Date Aug 3, 2022
Journal Biomolecules
Publisher MDPI
Peer Reviewed Peer Reviewed
Volume 12
Issue 8
Pages 1058
DOI https://doi.org/10.3390/biom12081058
Keywords Colon cancer; SEREX; Crohn’s-like lymphoid reaction (CLR); Immunotherapy; Immunoglobulin heavy chain
Public URL https://hull-repository.worktribe.com/output/4044340

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Publisher Licence URL
http://creativecommons.org/licenses/by/4.0

Copyright Statement
Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
This article is an open access article distributed under the terms and
conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).







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