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Platelet releasate promotes skeletal myogenesis by increasing muscle stem cell commitment to differentiation and accelerates muscle regeneration following acute injury (2018)
Journal Article
Scully, D., Sfyri, P., Verpoorten, S., Papadopoulos, P., Muñoz-Turrillas, M. C., Mitchell, R., Aburima, A., Patel, K., Gutiérrez, L., Naseem, K. M., & Matsakas, A. (2019). Platelet releasate promotes skeletal myogenesis by increasing muscle stem cell commitment to differentiation and accelerates muscle regeneration following acute injury. Acta Physiologica, 225(3), Article e13207. https://doi.org/10.1111/apha.13207

Aim The use of platelets as biomaterials has gained intense research interest. However, the mechanisms regarding platelet‐mediated skeletal myogenesis remain to be established. The aim of this study was to determine the role of platelet releasate in... Read More about Platelet releasate promotes skeletal myogenesis by increasing muscle stem cell commitment to differentiation and accelerates muscle regeneration following acute injury.

Effects of acute insulin-induced hypoglycaemia on endothelial microparticles in adults with and without type 2 diabetes (2018)
Journal Article
Al-Qaissi, A., Papageorgiou, M., Deshmukh, H., Madden, L. A., Rigby, A., Kilpatrick, E. S., Atkin, S. L., & Sathyapalan, T. (2019). Effects of acute insulin-induced hypoglycaemia on endothelial microparticles in adults with and without type 2 diabetes. Diabetes, Obesity and Metabolism, 21(3), 533-540. https://doi.org/10.1111/dom.13548

Aims Endothelial microparticles (EMPs) are novel surrogate markers of endothelial injury and dysfunction that may be differentially produced in response to acute insulin‐induced hypoglycaemia in adults with and without type 2 diabetes.

Agreement between blood draw techniques for assessing platelet activation by flow cytometry (2018)
Journal Article
Welch, E. L., Crooks, M. G., & Hart, S. P. (2019). Agreement between blood draw techniques for assessing platelet activation by flow cytometry. Platelets, 30(4), 530-534. https://doi.org/10.1080/09537104.2018.1535705

It is widely believed that assays of platelet activation are susceptible to preanalytical variables related to blood draw technique. We assessed platelet activation by whole blood flow cytometry and investigated the effects of: (1) drawing blood into... Read More about Agreement between blood draw techniques for assessing platelet activation by flow cytometry.

Inhibiting arginine methylation as a tool to investigate cross-talk with methylation and acetylation post-translational modifications in a glioblastoma cell line (2018)
Journal Article
Samuel, S. F., Marsden, A. J., Deepak, S., Rivero, F., Greenman, J., & Beltran-Alvarez, P. (2018). Inhibiting arginine methylation as a tool to investigate cross-talk with methylation and acetylation post-translational modifications in a glioblastoma cell line. Proteomes, 6(4), Article 44. https://doi.org/10.3390/proteomes6040044

Glioblastomas (GBM) are the most common grade 4 brain tumours; patients have very poor prognosis with an average survival of 15 months after diagnosis. Novel research lines have begun to explore aberrant protein arginine methylation (ArgMe) as a poss... Read More about Inhibiting arginine methylation as a tool to investigate cross-talk with methylation and acetylation post-translational modifications in a glioblastoma cell line.

Attenuation of oxidative stress-induced lesions in skeletal muscle in a mouse model of obesity-independent hyperlipidaemia and atherosclerosis through the inhibition of Nox2 activity (2018)
Journal Article
Sfyri, P. P., Yuldasheva, N. Y., Tzimou, A., Giallourou, N., Crispi, V., Aburima, A., Beltran-Alvarez, P., Patel, K., Mougios, V., Swann, J. R., Kearney, M. T., & Matsakas, A. (2018). Attenuation of oxidative stress-induced lesions in skeletal muscle in a mouse model of obesity-independent hyperlipidaemia and atherosclerosis through the inhibition of Nox2 activity. Free radical biology & medicine, 129, 504-519. https://doi.org/10.1016/j.freeradbiomed.2018.10.422

Obesity leading to hyperlipidaemia and atherosclerosis is recognised to induce morphological and metabolic changes in many tissues. However, hyperlipidaemia can occur in the absence of obesity. The impact of the latter scenario on skeletal muscle and... Read More about Attenuation of oxidative stress-induced lesions in skeletal muscle in a mouse model of obesity-independent hyperlipidaemia and atherosclerosis through the inhibition of Nox2 activity.

Phenotype-independent DNA methylation changes in prostate cancer (2018)
Journal Article
Pellacani, D., Droop, A. P., Frame, F. M., Simms, M. S., Mann, V. M., Collins, A. T., Eaves, C. J., & Maitland, N. J. (2018). Phenotype-independent DNA methylation changes in prostate cancer. The British Journal of Cancer, 119(9), 1133-1143. https://doi.org/10.1038/s41416-018-0236-1

Background Human prostate cancers display numerous DNA methylation changes compared to normal tissue samples. However, definitive identification of features related to the cells’ malignant status has been compromised by the predominance of cells wit... Read More about Phenotype-independent DNA methylation changes in prostate cancer.

A comprehensive targeted next-generation sequencing panel for genetic diagnosis of patients with suspected inherited thrombocytopenia (2018)
Journal Article
Johnson, B., Doak, R., Allsup, D., Astwood, E., Evans, G., Grimley, C., James, B., Myers, B., Stokley, S., Thachil, J., Wilde, J., Williams, M., Makris, M., Lowe, G. C., Wallis, Y., Daly, M. E., Morgan, N. V., & the UK GAPP Study Group. (2018). A comprehensive targeted next-generation sequencing panel for genetic diagnosis of patients with suspected inherited thrombocytopenia. Research and Practice in Thrombosis and Haemostasis, 2(4), 640-652. https://doi.org/10.1002/rth2.12151

Background: Inherited thrombocytopenias (ITs) are a heterogeneous group of disorders characterized by low platelet counts and often disproportionate bleeding with over 30 genes currently implicated. Previously the UK-GAPP study using whole exome sequ... Read More about A comprehensive targeted next-generation sequencing panel for genetic diagnosis of patients with suspected inherited thrombocytopenia.

Textural analysis demonstrates heterogeneous [18F]-fluorodeoxyglucose uptake in radiologically normal lung in patients with idiopathic pulmonary fibrosis (2018)
Journal Article
Aliyu, S. A., Avery, G., Cawthorne, C., Archibald, S. J., Kadir, T., Willaime, J. M., Morice, A. H., Hart, S. P., & Crooks, M. G. (2018). Textural analysis demonstrates heterogeneous [18F]-fluorodeoxyglucose uptake in radiologically normal lung in patients with idiopathic pulmonary fibrosis. European respiratory journal, 52(5), 1801138. https://doi.org/10.1183/13993003.01138-2018

Positron Emission Tomography (PET) scanning in idiopathic pulmonary fibrosis (IPF) has revealed increased [18F]-fluorodeoxyglucose ([18F]-FDG) uptake in areas of the lungs that appear normal on high resolution computed tomography (HRCT). We hypothesi... Read More about Textural analysis demonstrates heterogeneous [18F]-fluorodeoxyglucose uptake in radiologically normal lung in patients with idiopathic pulmonary fibrosis.

Amplified antimicrobial action of chlorhexidine encapsulated in PDAC-functionalized acrylate copolymer nanogel carriers (2018)
Journal Article
Al-Awady, M. J., Weldrick, P. J., Hardman, M. J., Greenway, G. M., & Paunov, V. N. (2018). Amplified antimicrobial action of chlorhexidine encapsulated in PDAC-functionalized acrylate copolymer nanogel carriers. Materials Chemistry Frontiers, 2(11), 2032-2044. https://doi.org/10.1039/c8qm00343b

We have developed and tested a novel functionalised nanocarrier for chlorhexidine (CHX) which provides a very strong enhancement of its antimicrobial action. The nanocarrier was based on lightly-cross-linked acrylate copolymer nanogel particles loade... Read More about Amplified antimicrobial action of chlorhexidine encapsulated in PDAC-functionalized acrylate copolymer nanogel carriers.

Glucose metabolism and metabolic flexibility in blood platelets (2018)
Journal Article
Aibibula, M., Naseem, K. M., & Sturmey, R. (2018). Glucose metabolism and metabolic flexibility in blood platelets. Journal of thrombosis and haemostasis : JTH, 16(11), 2300-2314. https://doi.org/10.1111/jth.14274

The metabolic integration processes required for platelet activation are unclear. The metabolic plasticity of human platelets were investigated. Activated platelets exhibit a glycolytic phenotype while preserving mitochondrial function. Platelets can... Read More about Glucose metabolism and metabolic flexibility in blood platelets.

Bisphosphonate Functionalized Gadolinium Oxide Nanoparticles Allow Long-Term MRI/CT Multimodal Imaging of Calcium Phosphate Bone Cement (2018)
Journal Article
Mastrogiacomo, S., Kownacka, A. E., Dou, W., Burke, B. P., de Rosales, R. T., Heerschap, A., Jansen, J. A., Archibald, S. J., & Walboomers, X. F. (2018). Bisphosphonate Functionalized Gadolinium Oxide Nanoparticles Allow Long-Term MRI/CT Multimodal Imaging of Calcium Phosphate Bone Cement. Advanced Healthcare Materials, 7(19), Article 1800202. https://doi.org/10.1002/adhm.201800202

Direct in vivo monitoring of bioconstructs using noninvasive imaging modalities such as magnetic resonance imaging (MRI) or computed tomography (CT) is not possible for many materials. Calcium phosphate–based composites (CPCs) that are applicable to... Read More about Bisphosphonate Functionalized Gadolinium Oxide Nanoparticles Allow Long-Term MRI/CT Multimodal Imaging of Calcium Phosphate Bone Cement.

CD28null pro-atherogenic CD4 T-cells explain the link between CMV infection and an increased risk of cardiovascular death (2018)
Journal Article
Pera, A., Caserta, S., Albanese, F., Blowers, P., Morrow, G., Terrazzini, N., Smith, H. E., Rajkumar, C., Reus, B., Msonda, J. R., Verboom, M., Hallensleben, M., Blasczyk, R., Davies, K. A., & Kern, F. (2018). CD28null pro-atherogenic CD4 T-cells explain the link between CMV infection and an increased risk of cardiovascular death. Theranostics, 8(16), 4509-4519. https://doi.org/10.7150/thno.27428

An increased risk of cardiovascular death in Cytomegalovirus (CMV)-infected individuals remains unexplained, although it might partly result from the fact that CMV infection is closely associated with the accumulation of CD28null T-cells, in particul... Read More about CD28null pro-atherogenic CD4 T-cells explain the link between CMV infection and an increased risk of cardiovascular death.

Assaying Rho GTPase–dependent processes in Dictyostelium discoideum (2018)
Journal Article
Marinovic, M., Xiong, H., Rivero Crespo, F., & Weber, I. (2018). Assaying Rho GTPase–dependent processes in Dictyostelium discoideum. Methods in molecular biology, 1821, 371-392. https://doi.org/10.1007/978-1-4939-8612-5_25

The model organism D. discoideum is well-suited to investigate basic questions of molecular and cell biology, particularly those related to the structure, regulation and dynamics of the cytoskeleton, signal transduction, cell-cell adhesion and develo... Read More about Assaying Rho GTPase–dependent processes in Dictyostelium discoideum.

Methods to study the roles of Rho GTPases in platelet function (2018)
Journal Article
Rivero Crespo, F., & Calaminus, S. (2018). Methods to study the roles of Rho GTPases in platelet function. Methods in molecular biology, 1821, 199-217. https://doi.org/10.1007/978-1-4939-8612-5_14

Platelets are a critical cell for prevention of bleeding. Part of the response to the formation of the thrombus is the activation of the actin cytoskeleton, with an inability to effectively activate the cytoskeleton linked to thrombus formation defec... Read More about Methods to study the roles of Rho GTPases in platelet function.

Methods to study the roles of Rho GTPases in platelet function (2018)
Book Chapter
Rivero, F., & Calaminus, S. (2018). Methods to study the roles of Rho GTPases in platelet function. In Methods in Molecular Biology; Rho GTPases (199-217). Humana Press. https://doi.org/10.1007/978-1-4939-8612-5_14

Platelets are a critical cell for prevention of bleeding. Part of the response to the formation of the thrombus is the activation of the actin cytoskeleton, with an inability to effectively activate the cytoskeleton linked to thrombus formation defec... Read More about Methods to study the roles of Rho GTPases in platelet function.

Increase of Direct C-C Coupling Reaction Yield by Identifying Structural and Electronic Properties of High-Spin Iron Tetra-azamacrocyclic Complexes (2018)
Journal Article
Brewer, S. M., Wilson, K. R., Jones, D. G., Reinheimer, E. W., Archibald, S. J., Prior, T. J., Ayala, M. A., Foster, A. L., Hubin, T. J., & Green, K. N. (2018). Increase of Direct C-C Coupling Reaction Yield by Identifying Structural and Electronic Properties of High-Spin Iron Tetra-azamacrocyclic Complexes. Inorganic chemistry, 57(15), 8890-8902. https://doi.org/10.1021/acs.inorgchem.8b00777

Macrocyclic ligands have been explored extensively as scaffolds for transition metal catalysts for oxygen and hydrogen atom transfer reactions. C–C reactions facilitated using earth abundant metals bound to macrocyclic ligands have not been well-unde... Read More about Increase of Direct C-C Coupling Reaction Yield by Identifying Structural and Electronic Properties of High-Spin Iron Tetra-azamacrocyclic Complexes.

The membrane-associated fraction of cyclase associated protein 1 translocates to the cytosol upon platelet stimulation (2018)
Journal Article
Joshi, P., Riley, D. R., Khalil, J. S., Xiong, H., Ji, W., & Rivero, F. (2018). The membrane-associated fraction of cyclase associated protein 1 translocates to the cytosol upon platelet stimulation. Scientific reports, 8, Article 10804. https://doi.org/10.1038/s41598-018-29151-w

Platelets undergo profound shape changes upon adhesion to damaged blood vessel walls that are mediated by reorganisation of the actin cytoskeleton in response to receptor-mediated signalling cascades. The highly conserved 56 kDa multidomain cyclase a... Read More about The membrane-associated fraction of cyclase associated protein 1 translocates to the cytosol upon platelet stimulation.

The membrane-associated fraction of cyclase associate protein 1 translocates to the cytosol upon platelet stimulation (2018)
Journal Article
Joshi, P., Riley, D. R., Khalil, J. S., Xiong, H., Ji, W., & Rivero, F. (2018). The membrane-associated fraction of cyclase associate protein 1 translocates to the cytosol upon platelet stimulation. Scientific reports, 8(1), Article 10804. https://doi.org/10.1038/s41598-018-29151-w

Platelets undergo profound shape changes upon adhesion to damaged blood vessel walls that are mediated by reorganisation of the actin cytoskeleton in response to receptor-mediated signalling cascades. The highly conserved 56 kDa multidomain cyclase a... Read More about The membrane-associated fraction of cyclase associate protein 1 translocates to the cytosol upon platelet stimulation.

Plastic scintillator-based microfluidic devices for miniaturized detection of Positron Emission Tomography radiopharmaceuticals (2018)
Journal Article
Tarn, M. D., Kızılyer, N. Y., Esfahani, M. M. N., Joshi, P., Brown, N. J., Pamme, N., Jenkins, D. G., & Archibald, S. J. (2018). Plastic scintillator-based microfluidic devices for miniaturized detection of Positron Emission Tomography radiopharmaceuticals. Chemistry: a European journal, 24(52), 13749-13753. https://doi.org/10.1002/chem.201802395

A miniaturized radio-HPLC detector has been developed comprising a microfluidic device fabricated from plastic scintillator in combination with a silicon photomultiplier light sensor, and tested with samples containing a positron-emitting radionuclid... Read More about Plastic scintillator-based microfluidic devices for miniaturized detection of Positron Emission Tomography radiopharmaceuticals.