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Dr Roger Sturmey


Measurement of Glutathione as a Tool for Oxidative Stress Studies by High Performance Liquid Chromatography (2020)
Journal Article
Nuhu, F., Gordon, A., Sturmey, R., Seymour, A. M., & Bhandari, S. (2020). Measurement of Glutathione as a Tool for Oxidative Stress Studies by High Performance Liquid Chromatography. Molecules, 25(18), https://doi.org/10.3390/molecules25184196

Background: Maintenance of the ratio of glutathione in the reduced (GSH) and oxidised (GSSG) state in cells is important in redox control, signal transduction and gene regulation, factors that are altered in many diseases. The accurate and reliable d... Read More about Measurement of Glutathione as a Tool for Oxidative Stress Studies by High Performance Liquid Chromatography.

Glucose concentration during equine in vitro maturation alters mitochondrial function (2020)
Journal Article
Lewis, N., Hinrichs, K., Leese, H. J., McGregor Argo, C., Brison, D. R., & Sturmey, R. G. (2020). Glucose concentration during equine in vitro maturation alters mitochondrial function. Reproduction, 160(2), 227-237. https://doi.org/10.1530/REP-20-0032

The use of in vitro embryo production in the horse is increasing in clinical and research settings, however, protocols are yet to be optimised. Notably, the two most commonly used base media for in vitro maturation (IVM) supply glucose at markedly di... Read More about Glucose concentration during equine in vitro maturation alters mitochondrial function.

Metabolic profile of in-vitro derived human embryos is not affected by the mode of fertilization (2020)
Journal Article
Sturmey, R. G., & Leary, C. (2020). Metabolic profile of in-vitro derived human embryos is not affected by the mode of fertilization. Molecular Human Reproduction, 26(4), 277-287. https://doi.org/10.1093/molehr/gaaa015

The pattern of metabolism by early embryos in vitro has been linked to a range of phenotypes, including viability. However, the extent to which metabolic function of embryos is modified by specific methods used during ART has yet to be fully describ... Read More about Metabolic profile of in-vitro derived human embryos is not affected by the mode of fertilization.

Spatial and Pregnancy-Related Changes in the Protein, Amino Acid, and Carbohydrate Composition of Bovine Oviduct Fluid (2020)
Journal Article
Rodríguez-Alonso, B., Maillo, V., Acuña, O. S., López-Úbeda, R., Torrecillas, A., Simintiras, C. A., …Rizos, D. (2020). Spatial and Pregnancy-Related Changes in the Protein, Amino Acid, and Carbohydrate Composition of Bovine Oviduct Fluid. International Journal of Molecular Sciences, 21(5), https://doi.org/10.3390/ijms21051681

Knowledge of how the biochemical composition of the bovine oviduct is altered due to the oviduct anatomy or the presence of an embryo is lacking. Thus, the aim of this study was to assess the effect of (І) oviduct anatomy and (ІІ) embryo presence on... Read More about Spatial and Pregnancy-Related Changes in the Protein, Amino Acid, and Carbohydrate Composition of Bovine Oviduct Fluid.

Good practice recommendations for the use of time-lapse technology† (2020)
Journal Article
Apter, S., Ebner, T., Freour, T., Guns, Y., Kovacic, B., Le Clef, N., …ESHRE Working group on Time-lapse technology, . (2020). Good practice recommendations for the use of time-lapse technology†. Human Reproduction Open, 2020(2), https://doi.org/10.1093/hropen/hoaa008

STUDY QUESTION: What recommendations can be provided on the approach to and use of time-lapse technology (TLT) in an IVF laboratory? SUMMARY ANSWER: The present ESHRE document provides 11 recommendations on how to introduce TLT in the IVF laboratory... Read More about Good practice recommendations for the use of time-lapse technology†.

Application of extracellular flux analysis for determining mitochondrial function in mammalian oocytes and early embryos (2019)
Journal Article
Muller, B., Lewis, N., Adeniyi, T., Leese, H. J., Brison, D. R., & Sturmey, R. G. (2019). Application of extracellular flux analysis for determining mitochondrial function in mammalian oocytes and early embryos. Scientific reports, 9, https://doi.org/10.1038/s41598-019-53066-9

Mitochondria provide the major source of ATP for mammalian oocyte maturation and early embryo development. Oxygen Consumption Rate (OCR) is an established measure of mitochondrial function. OCR by mammalian oocytes and embryos has generally been rest... Read More about Application of extracellular flux analysis for determining mitochondrial function in mammalian oocytes and early embryos.

Application of extracellular flux analysis for determining mitochondrial function in mammalian oocytes and early embryos (2019)
Working Paper
Muller, B., Lewis, N., Adeniyi, T., Leese, H. J., Brison, D., & Sturmey, R. G. Application of extracellular flux analysis for determining mitochondrial function in mammalian oocytes and early embryos

Background Mitochondria provide the major source of ATP for mammalian oocyte maturation and early embryo development. Oxygen Consumption Rate (OCR) is an established measure of mitochondrial function. OCR by mammalian oocytes and embryos has generall... Read More about Application of extracellular flux analysis for determining mitochondrial function in mammalian oocytes and early embryos.

Gene expression and metabolic response of bovine oviduct epithelial cells to the early embryo (2019)
Journal Article
Hamdi, M., Sánchez-Calabuig, M. J., Rodríguez-Alonso, B., Bagés-Arnal, S., Roussi, K., Sturmey, R., …Rizos, D. (2019). Gene expression and metabolic response of bovine oviduct epithelial cells to the early embryo. Reproduction, 158(1), 85-94. https://doi.org/10.1530/REP-18-0561

During its journey through the oviduct, the bovine embryo may induce transcriptomic and metabolic responses, via direct or indirect contact, from bovine oviduct epithelial cells (BOEC). An in vitro model using polyester mesh was established, allowing... Read More about Gene expression and metabolic response of bovine oviduct epithelial cells to the early embryo.

The enigmatic morula: mechanisms of development, cell fate determination, self-correction and implications for ART (2019)
Journal Article
Coticchio, G., Lagalla, C., Sturmey, R., Pennetta, F., & Borini, A. (2019). The enigmatic morula: mechanisms of development, cell fate determination, self-correction and implications for ART. Human Reproduction Update, 25(4), 422–438. https://doi.org/10.1093/humupd/dmz008

Background: Assisted reproduction technology offers the opportunity to observe the very early stages of human development. However, due to practical constraints, for decades morphological examination of embryo development has been undertaken at a few... Read More about The enigmatic morula: mechanisms of development, cell fate determination, self-correction and implications for ART.

Metabolomic Screening of Embryos to Enhance Successful Selection and Transfer (2019)
Book Chapter
McKeegan, P. J., & Sturmey, R. G. (2019). Metabolomic Screening of Embryos to Enhance Successful Selection and Transfer. In G. Kovacs, A. Rutherford, & D. K. Gardner (Eds.), How to prepare the egg and embryo to maximize IVF success, 295-304. Cambridge University Press. https://doi.org/10.1017/9781316756744.025

A key role of cellular metabolism is the provision of energy, in the form of adenosine triphosphate (ATP). In the case of the embryo, energy demand is dynamic and highly regulated to facilitate developmental milestones such as fertilization, cleavage... Read More about Metabolomic Screening of Embryos to Enhance Successful Selection and Transfer.

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