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Growth of MRSA and Pseudomonas aeruginosa in a fine-celled foam model containing sessile commensal skin bacteria

Oates, Angela; McBain, Andrew J.

Authors

Angela Oates

Andrew J. McBain



Abstract

Sessile cultures of the skin bacteria Staphylococcus saprophyticus and Corynebacterium xerosis were grown using novel fine-celled foam substrata to test the outcome of challenge by methicillin-resistant Staphylococcus aureus or Pseudomonas aeruginosa under three growth medium regimens (simulated sweat, simulated serum or simulated sweat substituted with simulated serum during the microbial challenge). S. saprophyticus and C. xerosis significantly limited MRSA and P. aeruginosa immigration respectively, under the simulated sweat and serum medium regimes. Under the substitution medium regime however, MRSA and P. aeruginosa integrated into pre-established biofilms to a significantly greater extent, attaining cell densities similar to the axenic controls. The outcome of challenge was influenced by the medium composition and test organism but could not be predicted based on planktonic competition assays or growth dynamics. Interactions between skin and wound isolates could be modelled using the fine-celled foam-based system. This model could be used to further investigate interactions and also in preclinical studies of antimicrobial wound care regimens.

Citation

Oates, A., & McBain, A. J. (2016). Growth of MRSA and Pseudomonas aeruginosa in a fine-celled foam model containing sessile commensal skin bacteria. Biofouling, 32(1), 25-33. https://doi.org/10.1080/08927014.2015.1117607

Journal Article Type Article
Acceptance Date Nov 4, 2015
Online Publication Date Jan 4, 2016
Publication Date Jan 2, 2016
Deposit Date Jan 15, 2019
Publicly Available Date Apr 1, 2021
Journal Biofouling
Print ISSN 0892-7014
Publisher Taylor and Francis
Peer Reviewed Peer Reviewed
Volume 32
Issue 1
Pages 25-33
DOI https://doi.org/10.1080/08927014.2015.1117607
Keywords Colonisation resistance; Skin; Wound; MRSA; Pseudomonas aeruginosa; Staphylococcus saprophyticus; Corynebacterium xerosis
Public URL https://hull-repository.worktribe.com/output/1215866
Publisher URL https://www.tandfonline.com/doi/full/10.1080/08927014.2015.1117607
Additional Information Peer Review Statement: The publishing and review policy for this title is described in its Aims & Scope.; Aim & Scope: http://www.tandfonline.com/action/journalInformation?show=aimsScope&journalCode=gbif20

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Copyright Statement
© 2016 The Author(s). Published by Taylor & Francis
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.






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