Growth of MRSA and Pseudomonas aeruginosa in a fine-celled foam model containing sessile commensal skin bacteria

Oates, Angela; McBain, Andrew J.

Abstract

Sessile cultures of the skin bacteria Staphylococcus saprophyticus and Corynebacterium xerosis were grown using novel fine-celled foam substrata to test the outcome of challenge by methicillin-resistant Staphylococcus aureus or Pseudomonas aeruginosa under three growth medium regimens (simulated sweat, simulated serum or simulated sweat substituted with simulated serum during the microbial challenge). S. saprophyticus and C. xerosis significantly limited MRSA and P. aeruginosa immigration respectively, under the simulated sweat and serum medium regimes. Under the substitution medium regime however, MRSA and P. aeruginosa integrated into pre-established biofilms to a significantly greater extent, attaining cell densities similar to the axenic controls. The outcome of challenge was influenced by the medium composition and test organism but could not be predicted based on planktonic competition assays or growth dynamics. Interactions between skin and wound isolates could be modelled using the fine-celled foam-based system. This model could be used to further investigate interactions and also in preclinical studies of antimicrobial wound care regimens.

Citation

Oates, A., & McBain, A. J. (2016). Growth of MRSA and Pseudomonas aeruginosa in a fine-celled foam model containing sessile commensal skin bacteria. Biofouling, 32(1), 25-33. https://doi.org/10.1080/08927014.2015.1117607

• Journal Article Type: Article
• Acceptance Date: Nov 4, 2015
• Online Publication Date: Jan 4, 2016
• Publication Date: Jan 2, 2016
• Deposit Date: Jan 15, 2019
• Publicly Available Date: Apr 1, 2021
• Journal: Biofouling
• Print ISSN: 0892-7014
• Publisher: Taylor and Francis
• Peer Reviewed: Peer Reviewed
• Volume: 32
• Issue: 1
• Pages: 25-33
• DOI: https://doi.org/10.1080/08927014.2015.1117607
• Keywords: Colonisation resistance; Skin; Wound; MRSA; Pseudomonas aeruginosa; Staphylococcus saprophyticus; Corynebacterium xerosis
• Public URL: https://hull-repository.worktribe.com/output/1215866
• Publisher URL: https://www.tandfonline.com/doi/full/10.1080/08927014.2015.1117607
• Additional Information: Peer Review Statement: The publishing and review policy for this title is described in its Aims & Scope.; Aim & Scope: http://www.tandfonline.com/action/journalInformation?show=aimsScope&journalCode=gbif20